ReSyn Biosciences

MagReSyn® NTA & NTA Screening Kit

MagReSyn® NTA microparticles consist of nitrilotriacetic acid (NTA) residues with a strong metal-chelating property. MagReSyn® NTA is pre-chelated with nickel ions for affinity purification of histidine-tagged proteins. The product has been engineered for exceptional specificity, delivering highly pure target proteins.
For rapid affinity purification of 6x-histidine-tagged proteins

Since different types of His-tagged proteins may have varying affinities for a particular metal ion, we also offer a His-tagged protein purification screening kit (MagReSyn® NTA-KIT) containing MagReSyn® NTA chelated with 4 alternate divalent metal ions (Ni2+, Cu2+, Co2+, Zn2+) to identify the most suitable metal ion for optimal purification of your particular target protein. MagReSyn® NTA Copper, -Cobalt and -Zinc are currently available in 2 ml product formats.

Support: Proprietary polymer microspheres containing iron oxide (magnetite)
Binding capacity: > 1.0 mg.ml-1 (His-GFP)
Bead size: ~5-10 µm
Formulation: 25 mg.ml-1 suspension in 20% ethanol

MagReSyn® SAX

MagReSyn® SAX (strong anion exchange) is a magnetic polymeric microparticle support designed for capture, purification and recovery of biomolecules such as proteins/peptides, enzymes, antibodies by exploiting differences in net charge to reversibly adsorb oppositely-charged molecules.
For rapid protein fractionation of proteins by ion exchange & concentration and clean-up of proteins from dilute solutions pre-MS analysis

MagReSyn® SAX is ideal for fractionation of complex biological mixtures (e.g. serum, plasma, urine, CSF, cell lysates, culture supernatants etc.) prior to analytical methods such as 2-dimensional electrophoresis, HPLC or Mass Spectrometry. The microparticles have an exceptional capacity allowing for experimental miniaturization and elution in minimal volume.

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Binding capacity: > 20 mg.ml-1 (BSA) per ml
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol

MagReSyn® Amine

Amine (NH2) functional microparticles may be used to physically adsorb biomolecules through ionic interaction, or they can be chemically functionalized for covalent coupling of biomolecules using a variety of bi-functional chemical coupling agents. The most frequently used coupling agents include glutaraldehyde and 1,4-butanediol diglycidyl ether.
Primary Amine functional support for ligand adsorption or chemical modification

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Binding capacity: > 20 mg.ml-1 (BSA)
Bead size: ~5-10 µm

MagReSyn® Carboxyl

MagReSyn® Carboxyl is a magnetic polymeric microparticles support designed for covalent immobilization of primary amine-containing ligands through carbodiimide-succinimidyl ester activation and subsequent amide bond formation. Various strategies for the activation of carboxylate particles have been documented, but the most commonly used method for coupling proteins and other primary amine-containing ligands is through activation by EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) in an aqueous reaction. This reaction can be either single-step or a two-step reaction requiring the addition of N-hydroxysuccinimide (NHS) or the more stable sulfo-NHS yielding NHS ester or sulfo NHS ester intermediates.
For covalent ligand immobilization via their primary amine residues

Please note that coupling of proteins through NHS-EDC activation can be tricky, requires fresh reagents, and is prone to interference from a range of routine buffers used in life-sciences. For simpler coupling of your ligand we would recommend using MagReSyn® Amine with glutaraldehyde or epoxide activation, or activation of MagReSyn® Carboxyl with commercial coupling reagents such as Anteobind™.

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Binding capacity: > 20 mg.ml-1 (BSA)
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol

MagReSyn® Streptavidin

MagReSyn® Streptavidin microparticles contain covalently coupled streptavidin. The porous nature of the polymer technology allows for exceptional streptavidin capacity, translating into increased capacity for the immobilization/capture of biotinylated biomolecules. The high streptavidin load allows the use of as little as 10 µl beads, improving experimental miniaturization for high-throughput automated analysis. Molecular applications of MagReSyn® Streptavidin microparticles include, protein immobilization and purification; nucleic acid capture assays and preparation of single-stranded DNA templates for e.g. SELEX and pyrosequencing. The immobilization of biotinylated antibodies may subsequently be used in e.g. immunoassay.
High capacity for the capture of biotinylated molecules

Support: Proprietary polymer microparticles containing iron oxide (magnetite) with immobilized Streptavidin
Binding capacity (DNA): > 30 000 pmoles.ml-1 (Biotinylated oligo, 25-mer)
Binding capacity (Protein): > 3 mg.ml-1 (Biotinylated IgG)
Bead size: ~5-10 µm average

MagReSyn® Protein A and A-MAX

Ultra-capacity Protein A for the affinity purification of antibodies and immunoprecipitation

MagReSyn® Protein A microparticles contain covalently coupled Protein A for highly specific single-step purification of IgG to >95% purity from whole serum samples. Protein A is a ~56 kDa surface protein originally isolated from the cell wall of the bacterium Staphylococcus aureus and is widely used for highly specific purification of IgG antibodies and for immunoprecipitation experiments. Protein A has 5 immunoglobulin binding domains folded into a three-helix bundle and specifically binds the Fc region on the heavy chain of the IgG class of antibodies. More specifically, Protein A binds with high affinity to specific subclasses of human, rabbit, mouse, guinea pig, cat and pig IgG. It does not react with human IgG3, IgD or IgE, mouse IgM, IgA or IgE, or goat, koala and llama IgG. The high recombinant Protein A content of MagReSyn® ensures a high immunoglobulin capacity allowing for experimental miniaturization.

Support: Proprietary polymer microparticles containing iron oxide (magnetite) with immobilized Protein A
Binding capacity: > 2.4 mg.ml-1 (Rabbit IgG)
Bead size: ~5-10 µm
Formulation: 15 mg.ml-1 suspension in Tris buffer

MagReSyn® Protein A MAX microparticles have been specially engineered for custom applications where maximum antibody binding is desired. This includes microplate applications where miniaturization and high-throughput automated analysis are required.

Binding capacity: > 4.8 mg.ml-1 (Rabbit IgG)
Bead size: ~5-10 µm
Formulation: 15 mg.ml-1 suspension in Tris buffer

MagReSyn® Protein G

MagReSyn® Protein G is a proprietary magnetic polymeric microparticle support that provides a simple and convenient method for highly specific capture of various immunoglobulins from biological samples. Recombinant Streptococcal Protein G (lacking the albumin binding region for increased immunoglobulin specificity) is covalently linked to the magnetic microparticles reducing leaching during your experimentation, and potentially stabilizing the Protein G for applications in non-standard conditions. Protein G binds to most mammalian immunoglobulins with greater affinity than Protein A, including human IgG3 and rat IgG2a. It does not react with human IgM, IgD or IgA.
High capacity Protein G for affinity purification of antibodies and immunoprecipitation.

The high recombinant Protein G content of MagReSyn® ensures a high immunoglobulin capacity allowing for experimental miniaturization. MagReSyn® Protein G microparticles have been engineered to be highly specific, providing single-step purification of IgG to >95% from whole serum samples.

Support: Proprietary polymer microparticles containing iron oxide (magnetite) with immobilized Protein G
Binding capacity: > 2.4 mg.ml-1 (Rabbit IgG)
Bead size: ~5-10 µm
Formulation: 15 mg.ml-1 suspension in Tris buffer

MagReSyn® Trypsin

MagReSyn® Trypsin microparticles consist of covalently immobilized sequencing grade trypsin (TPCK treated to prevent chymotryptic activity) for protein digestion prior to mass spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized trypsin is stabilized against denaturation and retains functionality under denaturing conditions (including the presence of urea and SDS) with limited trypsin autolysis, allowing for new automated and miniaturized digestion workflows, improving digestion reproducibility.  
For rapid, automated tryptic digestion of proteins for mass spectrometry sample preparation

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Digestion ratio: 20 µl (300 µg) microspheres per 50 µg protein sample
Bead size: ~5-10 µm
Formulation: 15 mg.ml-1 suspension in 50 mM acetic acid

MagReSyn® Chymotrypsin

MagReSyn® Chymotrypsin microparticles consist of covalently immobilized sequencing grade chymotrypsin (TLCK treated to prevent tryptic activity) for protein digestion prior to mass spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized chymotrypsin enables automated and miniaturized digestion workflows improving reproducibility. The enzyme cleaves at bulky hydrophobic residues thus providing complementary protein coverage as compared to magnetically immobilized trypsin digestion (see MagReSyn® Trypsin).
For rapid, automated chymotryptic digestion of proteins for mass spectrometry sample preparation

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Digestion ratio: 20 µl (200 µg) microparticles per 50 µg protein sample
Bead size: ~5-10 µm
Formulation: 10 mg.ml-1 suspension in 50 mM acetic acid