A new combination of chemical compounds could lead the way to more cost-effective stem cell culture
Human pluripotent stem cells (hPSCs) can infinitely self-renew and develop into all major cell types in the body, making them important for organ repair and replacement. But culturing them in large quantities can be expensive. Now, scientists at Japan’s Kyoto University, with colleagues in India and Iran, have developed a more cost-effective culture by using a new combination of chemical compounds.
Current culture systems need to contain components that can sustain hPSC self-renewal while preventing them from differentiating into other cell types. Of these components, genetically engineered growth factors produced in bacteria or animal cells, are particularly expensive.
The new culture was able to support and maintain the long-term renewal of hPSCs without the need for expensive growth factors.
Kouichi Hasegawa of Kyoto University’s Institute for Integrated Cell-Material Sciences (iCeMS) and his team developed their ‘AKIT’ culture using three chemical compounds: 1-azakenpaullone (AK), ID-8 (I), and tacrolimus (T).
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