Archive for Category: Tissue Dissociation

  • Our partner VitaCyte has published a great summary. It´s very helpful. So we like to share with you what their CEO BobMcCarthy wrote: More human islet isolations have been performed using purified collagenase and thermolysin than any other purified enzyme mixture (1). Several reports have shown the adverse effects when using thermolysin for human islet […]

    Thermolysin & Human Islet Isolation: Truth vs. Fiction

    Our partner VitaCyte has published a great summary. It´s very helpful. So we like to share with you what their CEO BobMcCarthy wrote: More human islet isolations have been performed using purified collagenase and thermolysin than any other purified enzyme mixture (1). Several reports have shown the adverse effects when using thermolysin for human islet isolation (2, 3). This blog post reviews the truth and fiction when using thermolysin for human islet isolation. It will also briefly discuss...
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  • FREE Webinar: Join us now! Date: Thu,  Oct  12, 2017 Time: 10 AM and  4 PM CET Reproducible from the start: How enzymes influence your data presented by Peter Frost, PhD, CEO, PELOBIOTECH   The success of cell isolation from solid tissues is dependent on the quality of the enzymes (collagenase and neutral protease) being […]

    Tissue Dissociation – Moving from a Black Box to Defined Enzyme Mixtures

    FREE Webinar: Join us now! Date: Thu,  Oct  12, 2017 Time: 10 AM and  4 PM CET Reproducible from the start: How enzymes influence your data presented by Peter Frost, PhD, CEO, PELOBIOTECH   The success of cell isolation from solid tissues is dependent on the quality of the enzymes (collagenase and neutral protease) being used. Since the 1960s crude collagenase products from Clostridium histolyticum have been used. The products are only minimal purified from c. Histolyticim...
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