• Akron Biotech webinar on “3D Nanofiber Scaffolds for Regenerative Medicine”

    In Allgemein, Webinar on
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  • Real-time Mycoplasma Detection: From a Safer Lab to Faster Cell Therapy Product Release

    In Cell Culture on

    It has been almost a year since we first wrote about the importance of controlling your cultures against the onset of Mycoplasma contamination, and the statements we made back then are just as – if not more – important right now. The statistics – that 25% of all cultures are contaminated with Mycoplasma, and the limitations – that Mycoplasma is very hard to detect with regular light microscopes – still hold. Between then and now, the scientific community has increasingly highlighted the importance of developing and validating new methods for the more efficient detection of Mycoplasma in cultures.

    The European Pharmacopiea 2.6.7 Mycoplasmas specifies the requirements for the validation of a nucleic acid-based test for Mycoplasmas. Because the potential consequences of Mycoplasma-contaminated cultures are significant, implementing rapid methods for the testing of cell therapy products can accelerate their validation and release, which is of significant therapeutic and commercial benefit.

    With this in mind, the European Medicines Agency (EMA) approved a real time polymerase chain reaction (Real-Time PCR) Mycoplasma test in June 2013 for the testing and release of a lot of matrix-applied characterized autologous cultured chondrocytes (MACI®) which were approved back in April of the same year.

    Mycoplasma RT-PCR detection systems are becoming more commonplace as an increasing number of scientific papers reports on their efficacy and speed. Regulatory agencies and validation programs are beginning to see the benefits that these detection systems bring and will begin incorporating them more thoroughly as part of release tests for cell therapy programs.

    On 9 December 2014, the European Compliance Academy is hosting a Mycoplasma Testing Conference in Heidelberg, Germany which will feature many prominent speakers and companies. The one-day meeting will highlight new technologies for the detection of Mycoplasmas and the current research in the area, where RT-PCR systems are expected to take a central role.

    With the above in mind, it is clear that containing Mycoplasma contamination is a critical aspect of cell therapy product development, and the awareness of the need for improved, more rapid detection approaches that will streamline the release process for cell therapies. We are curious to hear about your experiences with Mycoplasma contamination: do you think about it and how big of a part of your R&D/cell therapy process development scheme does it occupy? Write us or share your thoughts in the comments.

    Finally, don’t forget to check out Akron’s own, industry-leading Mycosolutions RT-PCR kit, which will be available the first week of October. (posted by AkronBiotech on September 29th 2014; http://blog.akronbiotech.com/)

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  • Angiogenesis Reagents

    In Angiogenesis on

    The V2a Kit™ – Vasculogenesis to Angiogenesis

    “An in vitro human model of angiogenesis that includes all the known phases of the angiogenic process”

    The V2a Kit contains all of the cells and reagents necessary to successfully complete a 24 well angiogenesis assay.

    • Designed to allow testing of compounds, media or tissue explants
    • 24 well format
    • Test compounds can be added at any time (from the onset of vasculogenesis through to advanced angiogenesis)
    • Analyse any aspect of the angiogenic pathway (cell activation, cell proliferation, migration,
      elongation and anastomosis) with complete flexibility of experimental timing

    For further information regarding the V2a Kit (Product Code ZHA-4000) please click here.

    Angiogenesis Tested Cells and Media for Bespoke Assay Design

    “Cellworks provide the cells, media and supporting reagents for bespoke angiogenesis assays” 

    Our angiogenesis tested cells allow flexibility in designing angiogenesis assays. They bring complete control over the number and cell types present in the assay, and the opportunity to manipulate the cells prior to culturing, for example by transfection.

    Supporting reagents including media, supplements, controls and tubule staining kits are all available.

    For further information about bespoke angiogenesis assay options, click here

    Angiogenesis Image Analysis Software

    “The AngioSys 2.0 image analysis software provides a semi-automated analysis of angiogenesis by measuring the number of tubules, the number of junctions, the total tubule length, and the mean tubule length for each image”

    • Custom built software developed to quantitate tubules formed during in vitro angiogenesis assays based on the co-culture of endothelial cells with fibroblasts.
    • Available in two formats:
      • 21 day demonstration version, product code ZHA-5000D: Download free of charge
      • Full licence, product code ZHA-5000: Activation of the full licence requires a serial key number which will be emailed upon receipt of a purchase order

    For further information about AngioSys Image Analysis Software, click here

    Angiogenesis Image Analysis Service

    ‘Ideal for customers preferring the in-house specialists at Cellworks to analyse their images. Customer reports detail the number of tubules, the number of junctions, the total tubule length, and the mean tubule length for each image’

    • Ideal for when analysis is required on only a small number of images
    • Suitable for when manpower in the lab is short
    • Perfect for customers new to angiogenesis image analysis

    For further information about the Angiogenesis Image Analysis Service Cellworks offer, click here

    What’s on your Angiogenesis ‘Wish List’?

    What’s missing from your Angiogenesis experiments? Recent customer feedback indicated the need for simple, accurate software to quantitate the number of tubules, and junctions in their angiogenesis images. We’ve delivered on this and have lots of happy customers. Our Product Managers would like to know what else you need, please email your thoughts to info@pelobiotech.com

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  • Upcoming webinar on 3D Cell Culture

    In Webinar on

    Introduction into 3D Cell Culture

     September 23,  2014 (Tuesday) at 10 AM (CEST) & September 25, 2014 (Thursday) at 4 PM (CEST)

    Speaker: Peter Frost, PELOBIOTECH
    Language: German

    We will give you introduction on different 3D systems and will present examples to demonstrate the high value of this technologies. (Scaffold based systems – Hydrogel based systems – Spheroid/Microtissues)

     

    Magnetic Levitation and 3D Bioprinting: Rapid 3D cell culture for high-throughput testing

    September 23, 2014 Thuesday at 3:30 PM (CEST)

    Speaker: Hubert Tseng, PhD
    Senior Scientist, Nano3D Biosciences
    Language: English

    In this webinar, an overview of the technology and published results will be discussed, and more recent innovations in high-throughput assays will be introduced.

     

    3D Nanofiber Scaffolds for Regenerative Medicine

    September 30, 2014 Tuesday at 4 PM (CEST) 

    Speaker: Sandro Matosevic, PhD
    Senior Scientist,  Akron Biotech
    Language: English

    In this webinar, an overview of nanofiber scaffolds for regenerative medicine application will be discussed.

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  • Towards ‘smarter’ 3D Scaffolds

    In 3D Cell Culture on

    Cells in 3D in vitro culture have many requirements concerning microenvirnonment. We mentioned alraedy the importance of stiffness and Akron has posted an interesting blog about 3D scaffolds. They focus on new smarter 3D scaffolds which are coated with growth factors or cells. Read more

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  • Microenvironment stiffness is an essential tool in stem cell differentiation

    The stiffness of the microenvironment has a significant influence on stem cell differentiation as show by Bioengineers at te University of California, San Diego. Stem cells grown in a hydrogel or on stiff materials very become bone-like-cells, whereas the usage of soft materials will  direct stem cells neural or fat cells.

    For more information please check out Adam Engler´s paper at  http://www.nature.com/nmat/journal/vaop/ncurrent/full/nmat4051.html

     

     

     

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  • We will give a lecture on 3D Cell Culture at the Live Cell Imaging Workshop of Olympus and ibidi in Vienna.

    In workshop on

    The workshop will be in cooperation with the core facility imaging
    on September 3rd 2013 – 10.00 am
    Anna Spiegel Forschungsgebäude (AKH Wien)- seminar room – level 03

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  • d-StemTM Tri-lineage

    In product news, Stem Cell Culture on
    • Analyze up to 24 hESC/iPSC clones simultaneously in 96 well format
    • Optimal Matrix for each germ layer differentiation
    • Complete formulated differentiation media
    • Validated antibodies: Sox1, Sox17 and Brachyury
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