ATCC recommend to use CryoStor for cryopreservation of hematopoietic stem cells and progenitor cellsweiter LesenBone Marrow CD34+ cells were cryopreserved using CryoStor CS 10 and had a viability of > 95 % after thawing.For further information just check the ATCC App Note:
- In Allgemein onweiter Lesen
Pelobiotech News 02/2015
our PELOBIOTECH News and other interesting news will be published also on our blog (www.pelobiotech.com/wordpress).
We are happy to introduce Maximiliane Kronberger to you. She joined our comapny recently and is in charge of all ReproCell Familiy products (ReproCell, Stemgent, Bioserve and Reinnervate).
Content New Products at a Glance
We acquired many new product lines and products to our One-Stop-Cell-Shop recently:
- Stemgent – ES/iPs Tools (reprogramming, self renewal and differentiation)
- Reinnervate – Scaffold-based 3D Cell Culture Systems up to 384 well plates
- Kaly-Cell – Human primary hepatocytes (fresh & cryopreserved)
- Bioserve – Human Tissue Acquisition (snap frozen tissue, FFPE block, serum, RNA and DNA) from healthy & diseased donors (cancer, metabolic deseases, immunological disorders and others)
Tools for iPS Cells (Reprogramming, Self Renewal and Differentiation)
Stemgent, Inc. is developing innovative technology and application solutions for the advancement of stem cell research. Stemgent products are designed by leading stem cell researchers worldwide.
Stemgent offers solutions for reprogramming, self renewal and differentiation. The product portfolio contains media, virus reprogramming tools, cytokines/proteins, small molecules and antibodies.
Human Hepatocytes (fresh & cryopreserved)
Kaly-Cell (Plobsheim, France) your partner in the field of hepatocytes.
We offer a selection of liver-derived products for drug metabolism-related research such as subcellular fractions, hepatocytes from human and toxicologically-relevant species i.e. monkey, minipig, dog, rat and mouse.
All products are offered at the same price as offered by Kayl-Cell.
Click here for more information on Human Hepatocytes products!
New Cell Counting Reagent
CellApplications, Inc launched Cyto-X a ready-for-use cell viability reagent for proliferation and cytotoxicity assays. It is a highly water-soluble tetrazolium salt which is reduced by dehydrogenases in live cells to form an orange colored formazan product that is soluble in culture medium. The amount of formazan product is directly proportional to the enzymatic activity of dehydrogenases and the number of living cells.
For further information just click here!
Deal of the Month
We will publish special promotions only via our “Deal of the Month” and only subscribers will have access.
To subscribe just click here.
- In Allgemein onweiter Lesen
Merry Christmas and a Happy New Year!
Christmas Wishes from everyone at PELOBIOTECH!
As we approach the end of 2014 we would like to take this opportunity to thank you for your business and support throughout 2014.
May your holiday season and the New Year be filled with much joy, happiness and success.
Our next Newsletter will be published in January.
Upcoming new products!
Human and animal Hepatocytes
Regrogramming Tools from StemGent
- In Cell Culture onweiter Lesen
It has been almost a year since we first wrote about the importance of controlling your cultures against the onset of Mycoplasma contamination, and the statements we made back then are just as – if not more – important right now. The statistics – that 25% of all cultures are contaminated with Mycoplasma, and the limitations – that Mycoplasma is very hard to detect with regular light microscopes – still hold. Between then and now, the scientific community has increasingly highlighted the importance of developing and validating new methods for the more efficient detection of Mycoplasma in cultures.
The European Pharmacopiea 2.6.7 Mycoplasmas specifies the requirements for the validation of a nucleic acid-based test for Mycoplasmas. Because the potential consequences of Mycoplasma-contaminated cultures are significant, implementing rapid methods for the testing of cell therapy products can accelerate their validation and release, which is of significant therapeutic and commercial benefit.
With this in mind, the European Medicines Agency (EMA) approved a real time polymerase chain reaction (Real-Time PCR) Mycoplasma test in June 2013 for the testing and release of a lot of matrix-applied characterized autologous cultured chondrocytes (MACI®) which were approved back in April of the same year.
Mycoplasma RT-PCR detection systems are becoming more commonplace as an increasing number of scientific papers reports on their efficacy and speed. Regulatory agencies and validation programs are beginning to see the benefits that these detection systems bring and will begin incorporating them more thoroughly as part of release tests for cell therapy programs.
On 9 December 2014, the European Compliance Academy is hosting a Mycoplasma Testing Conference in Heidelberg, Germany which will feature many prominent speakers and companies. The one-day meeting will highlight new technologies for the detection of Mycoplasmas and the current research in the area, where RT-PCR systems are expected to take a central role.
With the above in mind, it is clear that containing Mycoplasma contamination is a critical aspect of cell therapy product development, and the awareness of the need for improved, more rapid detection approaches that will streamline the release process for cell therapies. We are curious to hear about your experiences with Mycoplasma contamination: do you think about it and how big of a part of your R&D/cell therapy process development scheme does it occupy? Write us or share your thoughts in the comments.
Finally, don’t forget to check out Akron’s own, industry-leading Mycosolutions RT-PCR kit, which will be available the first week of October. (posted by AkronBiotech on September 29th 2014; http://blog.akronbiotech.com/)
- In Angiogenesis onweiter Lesen
The V2a Kit™ – Vasculogenesis to Angiogenesis
“An in vitro human model of angiogenesis that includes all the known phases of the angiogenic process”
The V2a Kit™ contains all of the cells and reagents necessary to successfully complete a 24 well angiogenesis assay.
- Designed to allow testing of compounds, media or tissue explants
- 24 well format
- Test compounds can be added at any time (from the onset of vasculogenesis through to advanced angiogenesis)
- Analyse any aspect of the angiogenic pathway (cell activation, cell proliferation, migration,
elongation and anastomosis) with complete flexibility of experimental timing
Angiogenesis Tested Cells and Media for Bespoke Assay Design
“Cellworks provide the cells, media and supporting reagents for bespoke angiogenesis assays”
Our angiogenesis tested cells allow flexibility in designing angiogenesis assays. They bring complete control over the number and cell types present in the assay, and the opportunity to manipulate the cells prior to culturing, for example by transfection.
Supporting reagents including media, supplements, controls and tubule staining kits are all available.
Angiogenesis Image Analysis Software
“The AngioSys 2.0 image analysis software provides a semi-automated analysis of angiogenesis by measuring the number of tubules, the number of junctions, the total tubule length, and the mean tubule length for each image”
- Custom built software developed to quantitate tubules formed during in vitro angiogenesis assays based on the co-culture of endothelial cells with fibroblasts.
- Available in two formats:
Angiogenesis Image Analysis Service
‘Ideal for customers preferring the in-house specialists at Cellworks to analyse their images. Customer reports detail the number of tubules, the number of junctions, the total tubule length, and the mean tubule length for each image’
- Ideal for when analysis is required on only a small number of images
- Suitable for when manpower in the lab is short
- Perfect for customers new to angiogenesis image analysis
What’s on your Angiogenesis ‘Wish List’?
What’s missing from your Angiogenesis experiments? Recent customer feedback indicated the need for simple, accurate software to quantitate the number of tubules, and junctions in their angiogenesis images. We’ve delivered on this and have lots of happy customers. Our Product Managers would like to know what else you need, please email your thoughts to email@example.com
- In Webinar onweiter Lesen
Introduction into 3D Cell Culture
September 23, 2014 (Tuesday) at 10 AM (CEST) & September 25, 2014 (Thursday) at 4 PM (CEST)
Speaker: Peter Frost, PELOBIOTECH
We will give you introduction on different 3D systems and will present examples to demonstrate the high value of this technologies. (Scaffold based systems – Hydrogel based systems – Spheroid/Microtissues)
Magnetic Levitation and 3D Bioprinting: Rapid 3D cell culture for high-throughput testing
September 23, 2014 Thuesday at 3:30 PM (CEST)
Speaker: Hubert Tseng, PhD
Senior Scientist, Nano3D Biosciences
In this webinar, an overview of the technology and published results will be discussed, and more recent innovations in high-throughput assays will be introduced.
3D Nanofiber Scaffolds for Regenerative Medicine
September 30, 2014 Tuesday at 4 PM (CEST)
Speaker: Sandro Matosevic, PhD
Senior Scientist, Akron Biotech
In this webinar, an overview of nanofiber scaffolds for regenerative medicine application will be discussed.
- In 3D Cell Culture onweiter Lesen
Cells in 3D in vitro culture have many requirements concerning microenvirnonment. We mentioned alraedy the importance of stiffness and Akron has posted an interesting blog about 3D scaffolds. They focus on new smarter 3D scaffolds which are coated with growth factors or cells. Read more
- weiter Lesen
The stiffness of the microenvironment has a significant influence on stem cell differentiation as show by Bioengineers at te University of California, San Diego. Stem cells grown in a hydrogel or on stiff materials very become bone-like-cells, whereas the usage of soft materials will direct stem cells neural or fat cells.
For more information please check out Adam Engler´s paper at http://www.nature.com/nmat/journal/vaop/ncurrent/full/nmat4051.html
We will give a lecture on 3D Cell Culture at the Live Cell Imaging Workshop of Olympus and ibidi in Vienna.In workshop onDownloadweiter Lesen
The workshop will be in cooperation with the core facility imaging
on September 3rd 2013 – 10.00 am
Anna Spiegel Forschungsgebäude (AKH Wien)- seminar room – level 03