• Munich Team won iGEM Award in US with 3D Printer

    In 3D Cell Culture, Allgemein on

    Congratulations to the Munich TU and LMU team: They won in Boston, US, the Bioengeeneering award iGEM in the category „Overgraduate“ . More here in German:

    Das Doppelteam von TU München und Ludwig-Maximilians-Universität (LMU) konnte mit einem 3D-Zelldrucker und passender Bio-Tinte die Fachjury am deutlichsten überzeugen. Mehr als 300 Hochschulteams aus aller Welt hatten beim „Giant Jamboree“ ihre Projekte zur Synthetischen Biologie präsentiert. Die deutschen Teams kehren mit reichlich Medaillen und Titeln von dem Megaevent an der US-Ostküste zurück: Für die 13 hiesigen Teams gab es fünfmal Gold, sechsmal Silber und zweimal Bronze.

    More here in German:

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  • Embryonic SCs and Fetal Tissue Research— will Trump Intervene?

    In Allgemein, Cell Culture on

    Of all the materials valued in biomedical research, embryonic stem (ES) cells and fetal tissue have gotten disproportionate attention from politicians. Because creating ES cell lines initially requires destroying a human embryo, President George W. Bush tightly restricted the use of federal funds for research on all but a few stem cell lines. President Barack Obama then made lifting those restrictions one of his first official actions after he took office in 2009.

    More recently, accusations that abortion clinics were unlawfully selling fetal tissue to researchers has stoked opposition to that type of research. So far, however, members of Congress have been unable to enact any restrictions into law.

    Now, biomedical researchers are wondering: How will a Donald J. Trump administration handle these ethically delicate materials?

    To read more about the concerns, please click here.

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  • New Product: Human iPSC-Derived Neural Stem Cells (i-HNSC)

    In Allgemein, product news on

    Human iPSC-Derived Neural Stem Cells (i-HNSC) are homogeneous, self-renewing and multipotent. Through partnership with leading stem cell company StemoniX, the cells are produced using next generation manufacturing technology. The result is large quantities of quality cells produced from each donor in a short period of time, cost effectively.

    Excellent Cell-Based Research Model

    • Neural Development
    • Neurotoxicity
    • Lineage Differentiation
    • Electrophysiology
    • Disease modeling
    • Drug discovery & development

    To learn more about this new product by our partner CellApplications  just give us a call or write an email to info@pelobiotech.com

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  • Wash your cloths, now: Dirty Laundry may cause Environmental Contamination

    In Allgemein on

    A new paper published in FEMS Microbiology Letters, resulting from an investigation of a laundry facility that services several Seattle-area hospitals, suggests that soiled clinical linens may be a source of surface Clostridium difficile contamination.

    C. difficile is a hospital and community acquired pathogen. C. difficile are spore-forming anaerobic bacteria that have been identified in 2-3% of healthy, non-hospitalized adults and in 10-25% of hospitalized adults. Toxin-producing C. difficile is the most common cause of hospital-acquired diarrhea. It is estimated that 25% of all C. difficile infections occur from exposures in the community that may stem from potential sources including water, soil, livestock, meats, vegetables and pets. There is evidence that C. difficile infections are seasonal and are correlated with 151 respiratory illnesses in the winter, due to antibiotic use.

    To read more, please clik here.

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  • Deep Insights: Let´s have a Look at the Marihuana Receptor

    In Allgemein on

    Crystal Structure of the Human Cannabinoid Receptor CB1

    Cannabinoid receptor 1 (CB1) is the principal target of Δ9-tetrahydrocannabinol (THC), a psychoactive chemical from Cannabis sativa with a wide range of therapeutic applications and a long history of recreational use. CB1 is activated by endocannabinoids and is a promising therapeutic target for pain management, inflammation, obesity, and substance abuse disorders. Here, we present the 2.8 Å crystal structure of human CB1 in complex with AM6538, a stabilizing antagonist, synthesized and characterized for this structural study. The structure of the CB1-AM6538 complex reveals key features of the receptor and critical interactions for antagonist binding. In combination with functional studies and molecular modeling, the structure provides insight into the binding mode of naturally occurring CB1 ligands, such as THC, and synthetic cannabinoids. This enhances our understanding of the molecular basis for the physiological functions of CB1 and provides new opportunities for the design of next-generation CB1-targeting pharmaceuticals.
    To read full article, please click here.

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  • FREE Webinar: Marvelous MSCs

    In Allgemein, Webinar on

    Marvelous MSCs: Tipps, Tricks and Tools for your MSC Cultures

    How MSC sourcing interact with your project.
    presented by Peter Frost , PhD, PELOBIOTECH, Germany
    Date: Th, Oct 25, 2016,
    Time: 10:00 AM or 3:00 PM (CET), language: German and English

    Mesenchymal stem cells (MSCs) are defined as multipotent stem cells being able for adipogenic, chondrogenic and osteogenic differentiation. Furthermore MSCs have to be positive for CD90, CD105 and CD73, and lacking the expression of CD14, CD34 and CD45.

    To sign up, please click here.

     

    Please share this information with your colleagues and friends.

    Thank you.

    Yopur PELOBIOTECH-Team

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  • PELOBiotech: EASY – New Endothelial Medium launched

    In Allgemein on

    We are happy to launch our EASY Endothelial Cell Growth Medium and offer you best value for the best price. We call this medium Easy, because it´s an easy choice – just get TOP quality for your cells at an irrestible TOP price.

    EASY has been developed for the special needs in basic research groups. “We use this easy-to use medium in our daily routine and are thrilled”, says our CSO, Dr. Lothar Steeb. “That´s why we decided to ease the access to it with an all-beating price.” It´s just so – EASY.

    The Cellovations® EASY Endothelial Cell Growth Medium is a complete medium formulated for optimal growth of endothelial cells out of large vessels. We want you to have a very convenient and easy opportunity to grow your cells with the best possible conditions and the best possible price. Due to the growing demand we can offer this premixed medium now at an irrestible price. Pay as low as 79 Euro p.Unit (500 ml).

    EASY contains

    • essential and non-essential amino acids,
    • vitamins,
    • organic and inorganic compounds,
    • hormones,
    • growth factors,
    • trace minerals
    • plus 2% FCS
    • together with the mixture of supplements.

    Stockpack price offers available.
    More information in our Newsletter or here.

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  • FREEZE: Have you met us? .. at IIR

    In Allgemein on

    Dresden – New ways and innovations in the field of CRYO-preservervation demonstrated Lothar Steeb, PhD and CSO PELOBIOTECH, at the IIR LS workshop in Dresden in September, 2016. Well visited was his latest workshop ‘New Protocols for Primary Cells and Reliable Results after Freezing’. “As biomedical research is focusing more and more on standardized cell culture under defined conditions, microenvironmental factors are getting more relevant for your cell culture work”, says Lothar Steeb. His presentation highlights some aspects which are necessary for a desirable high standard in cell culture in common as well as primary cell culture.

    To get the whole presentation please send an email with the titel ‘Cryo-Workshop’ to info@pelobiotech.com or give us a call: 0049 (0) 89 517 286 59 0.

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  • Tissue engineering: 3D Bioprinting Market is Growing Rapidly

    In 3D Cell Culture, Allgemein on

    3D bioprinting is fast becoming one of the most rapidly-growing areas of tissue engineering: the influx of new research, funds, patents and companies operating in the field is contributing to it becoming a multi-billion dollar business.

    While bioprinted organs are still not a reality, big steps towards making such pipe-dreams become clinical therapies are being made: an overview of the field was given in a recent opinion article published in Trends in Biotechnology by Dr. Ibrahim Ozbolat at the University of Iowa, which highlights the state of the art of the field and issues with the scale-up of functional tissue and organ constructs for transplantation.
    To read more please click here.

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  • The Promise of Cell Therapy in the Clinic: Can the Media be an Ally?

    In Allgemein on

    Even a cursory glance at general media over recent weeks would have indicated an increased amount of coverage dedicated to immunotherapy. Partly linked to recent developments in the filed, most major outlets have, in the past few months, chimed in, in one way or another, in a response to the major headlines.

    As an example, this week, the New York Times asked “What Is Immunotherapy?” in an article that followed on from recent NY Times reports commenting on the recent stem cell clinic data and the temporary halt of Juno Therapeutics’ CAR-T trials.

    Others have followed suit, reflecting what the media appears to perceive as a momentum that the field is currently enjoying. Yet there is optimistic caution, with the field and its treatments being called “controversial” in some reports. Such claims are understandable, especially due to the reactory nature with which the media operates.

    To read more please click here.

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  • Regenerative Medicine Improves Strength and Function in Muscle Injuries

    In Allgemein, regenerative medicine on

    Results of a study conducted by researchers at the University of Pittsburgh School of Medicine and the McGowan Institute for Regenerative Medicine showed significant improvement in strength and range of motion, as well as evidence for skeletal muscle regeneration, in 13 patients who were surgically implanted with bioscaffolds derived from pig tissue to treat muscle injuries. The patients had failed to respond to conventional treatment before use of the extracellular matrix (ECM).

    To read more, please click here.

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  • Controlled Release H2S Delivery System Protects graft Endothelium

    In Allgemein, Cell Culture on

    Hydrogen sulfide (H2S) functions as a protective gas transmitter in various physiological and pathological processes, but the lack of ideal donors severely hampers the clinical application of H2S. This study aims to construct a controlled release H2S donor and evaluate its protective effect on graft endothelium. Mesoporous silica nanoparticles (MSNs) were synthesized using the sol–gel method and loaded with diallyl trisulfide (DATS), an H2S-releasing agent named DATS-MSN. In vitro experiments showed that DATS-MSN could alleviate endothelial cell inflammation and enhance endothelial cell proliferation and migration. In vivo experiments demonstrated that the apoptosis of graft endothelium was mitigated in the presence of DATS-MSN. Our results indicated that DATS-MSN, releasing H2S in a controlled release fashion, could serve as an ideal H2S donor.

    To learn more, please click here.

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  • FREE WEBINAR: Check out the best Tumor Microenvironment Model

    In 3D Cell Culture, Allgemein, Webinar on

    It´s real and realistic. Find the best Tumor Microenvironment Model
    presented by Prabhakar Pandian, CTO, SynVivo, USA

    Date: Tue, July 19, 2016,
    Time: 3:30 PM (CET), English

    SynVivo-Tumor is a 3D cell-based assay for quantitative assessment in a physiologically realistic tumor microenvironment. The system enables real time visualization and analysis of cell-cell and cell-drug interactions encompassing
    – circulation in the microvasculature,
    – transport across the vessel walls,
    – delivery to the tumors.

    Applications
    There are a lot of areas of oncology research that can benefit by using the SynVivo-Tumor model. These include
    – basic research for understanding of the tumor microenvironment comprising of cell viability, proliferation, invasion and tumor-stromal and tumor-endothelium interactions;
    – drug delivery screening for efficacy and toxicity.
    Future applications are planned and furhter developments of the SynVivo Team will be highlighted in this webinar.

    To register for free, please click here.

     

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  • FREE WEBINAR: Endothelial Cell Culture

    Sensitive and Efficient Tools for Research  & Development
    presented by Lothar Steeb, CSO, PELOBIOTECH, Germany

    Date: Tue, June 28, 2016,
    Time: 10 AM (CET], language: German and 3 PM (CET), English

    To register for free, please click here.

    This webinar highlights different aspects of endothelial cell use and important improvement in cell cuture.

    You´ll  learn about:
    – isolation,
    – purification and
    – cultivation conditions.

     

    Please share this information with your colleagues  and friends. Thanks and have wonderful day

    Your PELOBiotech-Team

     

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  • PELOBIOTECH: Meet us at…

    In Allgemein on

    … Hausmesse in Bad Nauheim. The venue takes place
    Thur, June 09, 2016, 10:00 AM – 03:00 PM,
    at the foyer of the Max-Planck-Institut für Herz- und Lungenforschung. 

    You can inform yourself about hot topics of cryopreservation, brandnew media tools and procedures of genetic analyzing,  the new developments of digital PCR systems and our entire Primary Cell Portfolio esp. on The New Generation of 3D models.

    We love to keep in touch with you and cultivate the dialog with you.  Come along and meet our team and Dr. Lothar Steeb, CSO PELOBIOTECH.

     

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  • Differential Hypoxic Response of Human Choroidal and Retinal Endothelial cells Proposes Tissue Heterogeneity of Ocular Angiogenesis

    In Allgemein, Angiogenesis on

    Human CEC and REC were isolated by positive selection and were cultured. Characterization was performed by immunostaining for endothelial cell (EC)-specific markers. Total RNA and protein were extracted from normoxic or hypoxic CEC and REC cultures. Quantitative polymerase chain reaction (PCR) arrays were used to comparatively analyse 133 genes between CEC and REC, and the expression differences were calculated by ΔΔCt method. A total of 57 angiogenesis-related protein expression differences were investigated by Western blot and proteome profiler and were calculated by densitometry.

    Primary human CEC and REC lines stained positively for all EC markers and demonstrated high purity with similar staining and morphology. Under normoxia, CEC showed significantly lower expression levels for cell proliferation and vessel maturation genes and higher expression levels for inflammation-related genes when compared to REC. In response to hypoxia, CEC and REC displayed differential regulation for a multitude of angiogenesis-related genes and proteins. Furthermore, within the vascular endothelial growth factor (VEGF) family, CEC showed preferential upregulation for vascular endothelial growth factor A (VEGFA) while REC upregulated placenta growth factor (PlGF) levels.

    To read the conclusion, please click here.

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  • Scientists Discover how Zika virus Replicates in the Placenta

    In Allgemein, Cell Culture on

    Zika virus can infect and replicate in immune cells from the placenta, without killing them, scientists have discovered. The finding may explain how the virus can pass through the placenta of a pregnant woman, on its way to infect developing brain cells in her fetus.

    “Our results substantiate the limited evidence from pathology case reports,” says senior author Mehul Suthar, PhD, assistant professor of pediatrics at Emory University School of Medicine.. “It was known that the virus was getting into the placenta. But little was known about where the virus was replicating and in what cell type.”

    The results are published in Cell Host & Microbe. To read more please click here.

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  • GeneAll: New References

    In Allgemein on

    A disulphide-linked Heterodimer of TWIK-1 and TREK-1 mediates passive conductance in astrocytes

    TWIK-1 is a member of the two-pore domain K þ (K2P) channel family that plays an essential part in the regulation of resting membrane potential and cellular excitability. The physiological role of TWIK-1 has remained enigmatic because functional expression of TWIK-1 channels is elusive. Here we report that native TWIK-1 forms a functional channel at the plasma membrane of astrocytes. A search for TWIK-1-binding proteins led to the identification of TREK-1, another member of the K2P family. The TWIK-1/TREK-1 heterodimeric channel is formed via a disulphide bridge between residue C69 in TWIK-1 and C93 in TREK-1. Gene silencing demonstrates that surface expression of TWIK-1 and TREK-1 are interdependent. TWIK-1/TREK-1 heterodimers mediate astrocytic passive conductance and cannabinoid-induced glutamate release from astrocytes. Our study sheds new light on the diversity of K2P channels.

    To read more please click here, to find the reference please go to page 13.

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  • Scientists Identify ‘Smoking Gun’ in Metastasis of Hybrid Cells

    In Allgemein, Cell Culture on

    Cancer cells coordinate to form roving clusters

    Two-way communication between cancer cells appears to be key to their becoming motile, clustering and spreading through metastasis, according to Rice University scientists. Members of Rice’s Center for Theoretical Biological Physics have developed a model of how cancer cells twist a complex system of signals and feedback loops to their advantage. These signals help the cells detach from primary tumors and form clusters that lead to often-fatal metastatic disease.

    To read more, please click here.

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