• PELOBiotech: EASY – New Endothelial Medium launched

    In Allgemein on

    We are happy to launch our EASY Endothelial Cell Growth Medium and offer you best value for the best price. We call this medium Easy, because it´s an easy choice – just get TOP quality for your cells at an irrestible TOP price.

    EASY has been developed for the special needs in basic research groups. “We use this easy-to use medium in our daily routine and are thrilled”, says our CSO, Dr. Lothar Steeb. “That´s why we decided to ease the access to it with an all-beating price.” It´s just so – EASY.

    The Cellovations® EASY Endothelial Cell Growth Medium is a complete medium formulated for optimal growth of endothelial cells out of large vessels. We want you to have a very convenient and easy opportunity to grow your cells with the best possible conditions and the best possible price. Due to the growing demand we can offer this premixed medium now at an irrestible price. Pay as low as 79 Euro p.Unit (500 ml).

    EASY contains

    • essential and non-essential amino acids,
    • vitamins,
    • organic and inorganic compounds,
    • hormones,
    • growth factors,
    • trace minerals
    • plus 2% FCS
    • together with the mixture of supplements.

    Stockpack price offers available.
    More information in our Newsletter or here.

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  • FREEZE: Have you met us? .. at IIR

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    Dresden – New ways and innovations in the field of CRYO-preservervation demonstrated Lothar Steeb, PhD and CSO PELOBIOTECH, at the IIR LS workshop in Dresden in September, 2016. Well visited was his latest workshop ‘New Protocols for Primary Cells and Reliable Results after Freezing’. “As biomedical research is focusing more and more on standardized cell culture under defined conditions, microenvironmental factors are getting more relevant for your cell culture work”, says Lothar Steeb. His presentation highlights some aspects which are necessary for a desirable high standard in cell culture in common as well as primary cell culture.

    To get the whole presentation please send an email with the titel ‘Cryo-Workshop’ to info@pelobiotech.com or give us a call: 0049 (0) 89 517 286 59 0.

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  • Tissue engineering: 3D Bioprinting Market is Growing Rapidly

    In 3D Cell Culture, Allgemein on

    3D bioprinting is fast becoming one of the most rapidly-growing areas of tissue engineering: the influx of new research, funds, patents and companies operating in the field is contributing to it becoming a multi-billion dollar business.

    While bioprinted organs are still not a reality, big steps towards making such pipe-dreams become clinical therapies are being made: an overview of the field was given in a recent opinion article published in Trends in Biotechnology by Dr. Ibrahim Ozbolat at the University of Iowa, which highlights the state of the art of the field and issues with the scale-up of functional tissue and organ constructs for transplantation.
    To read more please click here.

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  • The Promise of Cell Therapy in the Clinic: Can the Media be an Ally?

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    Even a cursory glance at general media over recent weeks would have indicated an increased amount of coverage dedicated to immunotherapy. Partly linked to recent developments in the filed, most major outlets have, in the past few months, chimed in, in one way or another, in a response to the major headlines.

    As an example, this week, the New York Times asked “What Is Immunotherapy?” in an article that followed on from recent NY Times reports commenting on the recent stem cell clinic data and the temporary halt of Juno Therapeutics’ CAR-T trials.

    Others have followed suit, reflecting what the media appears to perceive as a momentum that the field is currently enjoying. Yet there is optimistic caution, with the field and its treatments being called “controversial” in some reports. Such claims are understandable, especially due to the reactory nature with which the media operates.

    To read more please click here.

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  • Regenerative Medicine Improves Strength and Function in Muscle Injuries

    In Allgemein, regenerative medicine on

    Results of a study conducted by researchers at the University of Pittsburgh School of Medicine and the McGowan Institute for Regenerative Medicine showed significant improvement in strength and range of motion, as well as evidence for skeletal muscle regeneration, in 13 patients who were surgically implanted with bioscaffolds derived from pig tissue to treat muscle injuries. The patients had failed to respond to conventional treatment before use of the extracellular matrix (ECM).

    To read more, please click here.

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  • Controlled Release H2S Delivery System Protects graft Endothelium

    In Allgemein, Cell Culture on

    Hydrogen sulfide (H2S) functions as a protective gas transmitter in various physiological and pathological processes, but the lack of ideal donors severely hampers the clinical application of H2S. This study aims to construct a controlled release H2S donor and evaluate its protective effect on graft endothelium. Mesoporous silica nanoparticles (MSNs) were synthesized using the sol–gel method and loaded with diallyl trisulfide (DATS), an H2S-releasing agent named DATS-MSN. In vitro experiments showed that DATS-MSN could alleviate endothelial cell inflammation and enhance endothelial cell proliferation and migration. In vivo experiments demonstrated that the apoptosis of graft endothelium was mitigated in the presence of DATS-MSN. Our results indicated that DATS-MSN, releasing H2S in a controlled release fashion, could serve as an ideal H2S donor.

    To learn more, please click here.

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  • FREE WEBINAR: Check out the best Tumor Microenvironment Model

    In 3D Cell Culture, Allgemein, Webinar on

    It´s real and realistic. Find the best Tumor Microenvironment Model
    presented by Prabhakar Pandian, CTO, SynVivo, USA

    Date: Tue, July 19, 2016,
    Time: 3:30 PM (CET), English

    SynVivo-Tumor is a 3D cell-based assay for quantitative assessment in a physiologically realistic tumor microenvironment. The system enables real time visualization and analysis of cell-cell and cell-drug interactions encompassing
    – circulation in the microvasculature,
    – transport across the vessel walls,
    – delivery to the tumors.

    Applications
    There are a lot of areas of oncology research that can benefit by using the SynVivo-Tumor model. These include
    – basic research for understanding of the tumor microenvironment comprising of cell viability, proliferation, invasion and tumor-stromal and tumor-endothelium interactions;
    – drug delivery screening for efficacy and toxicity.
    Future applications are planned and furhter developments of the SynVivo Team will be highlighted in this webinar.

    To register for free, please click here.

     

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  • FREE WEBINAR: Endothelial Cell Culture

    Sensitive and Efficient Tools for Research  & Development
    presented by Lothar Steeb, CSO, PELOBIOTECH, Germany

    Date: Tue, June 28, 2016,
    Time: 10 AM (CET], language: German and 3 PM (CET), English

    To register for free, please click here.

    This webinar highlights different aspects of endothelial cell use and important improvement in cell cuture.

    You´ll  learn about:
    – isolation,
    – purification and
    – cultivation conditions.

     

    Please share this information with your colleagues  and friends. Thanks and have wonderful day

    Your PELOBiotech-Team

     

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  • PELOBIOTECH: Meet us at…

    In Allgemein on

    … Hausmesse in Bad Nauheim. The venue takes place
    Thur, June 09, 2016, 10:00 AM – 03:00 PM,
    at the foyer of the Max-Planck-Institut für Herz- und Lungenforschung. 

    You can inform yourself about hot topics of cryopreservation, brandnew media tools and procedures of genetic analyzing,  the new developments of digital PCR systems and our entire Primary Cell Portfolio esp. on The New Generation of 3D models.

    We love to keep in touch with you and cultivate the dialog with you.  Come along and meet our team and Dr. Lothar Steeb, CSO PELOBIOTECH.

     

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  • Differential Hypoxic Response of Human Choroidal and Retinal Endothelial cells Proposes Tissue Heterogeneity of Ocular Angiogenesis

    In Allgemein, Angiogenesis on

    Human CEC and REC were isolated by positive selection and were cultured. Characterization was performed by immunostaining for endothelial cell (EC)-specific markers. Total RNA and protein were extracted from normoxic or hypoxic CEC and REC cultures. Quantitative polymerase chain reaction (PCR) arrays were used to comparatively analyse 133 genes between CEC and REC, and the expression differences were calculated by ΔΔCt method. A total of 57 angiogenesis-related protein expression differences were investigated by Western blot and proteome profiler and were calculated by densitometry.

    Primary human CEC and REC lines stained positively for all EC markers and demonstrated high purity with similar staining and morphology. Under normoxia, CEC showed significantly lower expression levels for cell proliferation and vessel maturation genes and higher expression levels for inflammation-related genes when compared to REC. In response to hypoxia, CEC and REC displayed differential regulation for a multitude of angiogenesis-related genes and proteins. Furthermore, within the vascular endothelial growth factor (VEGF) family, CEC showed preferential upregulation for vascular endothelial growth factor A (VEGFA) while REC upregulated placenta growth factor (PlGF) levels.

    To read the conclusion, please click here.

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  • Scientists Discover how Zika virus Replicates in the Placenta

    In Allgemein, Cell Culture on

    Zika virus can infect and replicate in immune cells from the placenta, without killing them, scientists have discovered. The finding may explain how the virus can pass through the placenta of a pregnant woman, on its way to infect developing brain cells in her fetus.

    “Our results substantiate the limited evidence from pathology case reports,” says senior author Mehul Suthar, PhD, assistant professor of pediatrics at Emory University School of Medicine.. “It was known that the virus was getting into the placenta. But little was known about where the virus was replicating and in what cell type.”

    The results are published in Cell Host & Microbe. To read more please click here.

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  • GeneAll: New References

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    A disulphide-linked Heterodimer of TWIK-1 and TREK-1 mediates passive conductance in astrocytes

    TWIK-1 is a member of the two-pore domain K þ (K2P) channel family that plays an essential part in the regulation of resting membrane potential and cellular excitability. The physiological role of TWIK-1 has remained enigmatic because functional expression of TWIK-1 channels is elusive. Here we report that native TWIK-1 forms a functional channel at the plasma membrane of astrocytes. A search for TWIK-1-binding proteins led to the identification of TREK-1, another member of the K2P family. The TWIK-1/TREK-1 heterodimeric channel is formed via a disulphide bridge between residue C69 in TWIK-1 and C93 in TREK-1. Gene silencing demonstrates that surface expression of TWIK-1 and TREK-1 are interdependent. TWIK-1/TREK-1 heterodimers mediate astrocytic passive conductance and cannabinoid-induced glutamate release from astrocytes. Our study sheds new light on the diversity of K2P channels.

    To read more please click here, to find the reference please go to page 13.

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  • Scientists Identify ‘Smoking Gun’ in Metastasis of Hybrid Cells

    In Allgemein, Cell Culture on

    Cancer cells coordinate to form roving clusters

    Two-way communication between cancer cells appears to be key to their becoming motile, clustering and spreading through metastasis, according to Rice University scientists. Members of Rice’s Center for Theoretical Biological Physics have developed a model of how cancer cells twist a complex system of signals and feedback loops to their advantage. These signals help the cells detach from primary tumors and form clusters that lead to often-fatal metastatic disease.

    To read more, please click here.

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  • International Stem Cell Guidelines Revised, Expanded

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    Widely recognized stem cell research guidelines now address controversial topics such as human gene editing.

    The decision by the International Society for Stem Cell Research to recommend that stem cell oversight bodies should review all embryo research comes from a recognition of rapidly advancing technologies that could make unprecedented advances in understanding human disease but also have ethical implications.

    “We in the scientific community have to be vigilant—and also transparent—about the way we do the research,” George Q. Daley, a Harvard University stem cell scientist and ISSCR board member who helped craft the new guidelines. The document is titled Guidelines for Stem Cell Science and Clinical Translation.

    To find out more, please clikc here.
    Download: ISSCR  guidelines.

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  • PELOBIOTECH: Meet us at… Analytica in Munich

    In Allgemein on

    Analytica is the International Trade Fair for Laboratory Technology, Analysis and Biotechnology. It´s meeting the 25th time this year from May 10-13, 2016, opening at 9 AM, in Munich. So meet us at our partner´s booth baseclick in Hall A3, booth A3, booth #313.

    Its unique exhibition profile unites all topics that pertain to modern analysis in research and industry. The various exhibition sectors will give you a comprehensive overview of the market.

    Download here the floorplan.

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  • S-stamp Marking Technique helps prevent Inverted Graft Implantation in DMEK

    In Allgemein, Cell Culture on

    Endothelial cell loss, re-bubbling and complication rates were similar in stamped and unstamped graft groups.

    A novel S-stamp marking method eliminated upside-down graft implantation in Descemet’s membrane endothelial keratoplasty, according to a study.

    The technique may help surgeons perfect the DMEK technique and avoid complications, according to Peter B. Veldman, MD, the corresponding author. To read more go to healio.

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  • FREE WEBINAR: Brandnew Media for Keratinocytes

    In Allgemein, Cell Culture, Webinar on

    Why, How and when is the Best Time to Switch to this Serum-free and Defined Media.

    presented by Lothar Steeb, PhD, CSO, PELOBIOTECH

    1. Tue, May 3, 2016 3:00 PM – 4:00 PM CEST (English)

      DEVELOPED BY PELOBIOTECH - FOR YOU!

      DEVELOPED BY PELOBIOTECH – FOR YOU!

    Please sign up and share this Information with your co-workers and friends. Thank you.

    Your PELOBiotech-Team

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  • Gene Editing Technique Improves Vision in Rats with Inherited Blindness

    In Allgemein, regenerative medicine on

    A Cedars-Sinai study has revealed that a research team at the Cedars-Sinai Board of Governors Regenerative Medicine Institute has discovered a new technique that has the potential to treat inherited diseases by removing genetic defects has been shown for the first time to hinder retinal degeneration in rats with a type of inherited blindness.

    The focus of the researchers was on inherited retinitis pigmentosa – a degenerative eye disease with no known cure that can lead to blindness. Â A technique called CRISPR/Cas9 was used by the researchers to remove a genetic mutation that causes the blindness disease.

    The technique CRISPR/Cas9 (CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeat, the type of DNA sequences involved in this process) is adapted from a strategy used by bacteria to fight invading viruses. The study was carried out in mice, however the finding are significant as they have potential implication sin humans as well. The details of the study findings were published recently in the journal Molecular Therapy.
    To read more, please click here.

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  • Researchers Create Micro Heart Muscle From Stem Cells

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    The medical research field has been looking into creating better solutions for delicate yet complex procedures that require utmost precision when dealing with heart problems and stem cell therapy is one of the possible solutions for treating heart failure.

    Luckily, Gladstone Institutes, whose mission is to overcome major unsolved diseases, has just given coronary disease researchers an excellent tool to advance methods and medicines for heart problems with its latest breakthrough.

    To read more please click here.

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