• Kinetics of circulating EPCs

    In Allgemein on

    Endothelial progenitor cells (EPCs) are primitive cells found in the bone marrow and peripheral blood (PB). In particular, the potential of EPCs to differentiate into mature endothelial cells remains of high interest for clinical applications such as bio-functionalized patches for autologous seeding after implantation. The objective of this study was to determine EPCs’ kinetics in patients undergoing carotid artery thromboendarterectomy (CTEA) and patch angioplasty.
    Methods: Twenty CTEA patients were included (15 male, mean age 76 years). PB samples were taken at 1 day preoperatively, and at 1, 3, and 5 days postoperatively. Flow cytometric analysis was performed for CD34, CD133, KDR, and CD45. Expression of KDR, SDF-1α, and G-CSF was analyzed by means of enzyme-linked immunosorbent assay.
    Results: Fluorescence-activated cell sorting analysis revealed 0.031%±0.016% (% of PB mononuclear cells) KDR+ cells and 0.052%±0.022% CD45-/CD34+/CD133+ cells, preoperatively. A 33% decrease of CD45–/CD34+/CD133+ cells was observed at day 1 after surgery. However, a relative number (compared to initial preoperative values) of CD45-/CD34+/CD133+ cells was found on day 3 (82%) and on day 5 (94%) postoperatively. More profound upregulated levels of CD45–CD34+/CD133+ cells were observed for diabetic (+47% compared to nondiabetic) and male (+38% compared to female) patients. No significant postoperative time-dependent differences were found in numbers of KDR+ cells and the concentrations of the cytokines KDR and G-CSF. However, the SDF-1α levels decreased significantly on day 1 postoperatively but returned to preoperative levels by day 3.
    Conclusion: CTEA results in short-term downregulation of circulating EPCs and SDF-1α levels. Rapid return to baseline levels might indicate participation of EPCs in repair mechanisms following vascular injury.

    To read the full article please click here.

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  • Astrocytes and Exosomes Implicated in Protein Propagation

    In Allgemein on

    Many researchers now believe that misfolded proteins spread from cell to cell across the brain, corrupting normal proteins as they go, yet exactly how this propagation would happen remains unclear. At the Society for Neuroscience annual meeting  scientists identified specific mechanisms that may be involved in the spread of pathological proteins in Parkinson’s and Alzheimer’s diseases. They argued that astrocytes transmit α-synuclein aggregates by cell-to-cell contact; for tau, they argued that its trans-synaptic spread depends on the secretory vesicles known as exosomes. In addition, researchers debuted a new method for following tau aggregates over time in living mouse brain.

    “Up until a few years ago, we did not understand how Parkinson’s disease progresses at the molecular level,” noted Alice Chen-Plotkin of the University of Pennsylvania. “Now, we may be starting to discover how the disease worsens, which could give insights into ways to slow it down or stop it,” she wrote to Alzforum.

    To read more, please click here.

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  • New clues to Myelination could help Identify ways to Intervene in Neurodegenerative Diseases

    In Allgemein on
    “If we can further enhance our understanding of how these oligodendrocyte precursor cells mature, then it may be possible to stimulate them to replace myelin in diseases like multiple sclerosis.”, says Pablo Paez, PhD, Assistant professor and research scientist , unters James Kelly Research Institute at University at Buffalo, US.

     The Researchers at the University at Buffalo have identified a critical step in myelination after birth that has significance for treating neurodegenerative diseases like multiple sclerosis, in which myelin is lost or damaged. Myelin is the protective coating that neurons need to function. The preclinical research  concerns oligodendrocytes, the cells that make myelin, and the progenitor cells that are their precursors. The work involved the study of voltage-operated calcium channels, which are responsible for initiating many physiological functions.

    To read more how a certain type of calcium channel is critical and may lead to improved therapies for diseases like multiple sclerosis please click here.

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  • Biopreservation Market is getting More and More Important

    In Allgemein, regenerative medicine on

    Increasing demand for preserving stem cells and rise in the number of egg & sperm banks along with the growing implementation of assisted reproductive technology (ART) across the globe is expected to boost the Biopreservation market demand over the coming years. Growing healthcare awareness among the people as well as technological breakthroughs in healthcare are predicted to complement the industry growth.

    A large global population suffering from lifestyle related diseases as well as cardiovascular disorders, high blood pressure, and cancer is predicted to promote the expansion of the market over the coming eight years. The increasing popularity of regenerative drugs along with wide biobanking application for cell, organ, and tissue preservation is further predicted to benefit the industry in near future. According to Global Market Insights Inc., “The biopreservation market size worth USD 3 billion in 2015, is predicted to exceed USD 9.7 billion mark, growing at a CAGR of 13.4% over the period of 2016-2024.” To read more, please click here.

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  • For Stem Cells to Be Healthy, Telomere Length Has to Be Just Right

    In Allgemein, regenerative medicine, Stem Cells on

    Ever since researchers connected the shortening of telomeres–the protective structures on the ends of chromosomes–to ageing and disease, the race has been on to understand the factors that govern telomere length.

    Now, scientists at the Salk Institute have found that a balance of elongation and trimming in stem cells results in telomeres that are, as Goldilocks would say, not too short and not too long, but just right.

    The finding, which appears in the December, 2016, issue of Nature Structural & Molecular Biology, deepens our understanding of stem cell biology and could help advance stem cell-based therapies, especially related to ageing and regenerative medicine.

    To read more please click here.

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  • Cell Signalling in the Cardiovascular System

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    The cardiovascular system is a highly complex, well organised system in which signal transduction plays critical physiological and pathophysiological roles. The cellular elements of the heart and vascular wall are equipped with an array of specific receptors and with complex intracellular machinery that facilitates and drives appropriate responses to extracellular stimuli. Understanding the mechanisms through which extracellular stimuli modify the functions of cells in the heart and vascular wall gives valuable insights into how perturbations of signalling systems can cause pathological situations. This knowledge will allow the identification of novel molecular targets for pharmacological intervention and will assist the future development of therapeutic strategies for managing cardiovascular disorders. This brief review will give a general overview of some major intracellular signalling systems operative in cells comprising the heart and vasculature, with particular emphasis on the pleiotropic roles of protein kinases as regulators of cell behaviour.

    To read this overview, please click here.

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  • Gut Tissue with Functional Nervous System Created in Laboratory for First Time

    In Allgemein, iPS media, regenerative medicine on

    Scientists created human gut tissue containing a functional nervous system in the laboratory for the first time.

    According to the authors of the study, “Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system,” which was published in the leading scientific journal Nature Medicine, this research could help treat people with conditions such as inflammatory bowel disease (IBD). However, they warned: “The complexity of the [gut] tissue will demand much more work before it reaches prime time in patients.”

    The team of researchers, led by James Wells, PhD, of the Division of Developmental Biology at Cincinnati Children’s Hospital Medical Center in Ohio, USA used a tissue engineering approach with embryonic stem (ES) cells and another type of stem cells called induced pluripotent stem cells (IPS) derived from either skin or white blood cells, to generate the human gut tissue.

    Read more here or the official statement.
    You can also watch the following YouTube here.

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  • Defined and Serum-free Media on the Rise

    In Allgemein on

    The global cell culture market has been broadly segmented into media, sera, and reagents. Chemically-defined media rarely provide desired yield, and thus the inclination toward more complex media increases. The adoption of chemically-defined media has been slow yet steady for both media manufacturers and bioprocessors. Demand for media has been shifting from serum-based to serum-sparing, serum-free animal derived, and chemically-defined media. Lysogeny broth (LB) is a nutritive medium used for artificial bacteria growth.

    The attachment factors segment accounted for the largest share of more than 25% of the global cell culture reagents market in 2014. However, amino acids is estimated to be the fastest growing segment during the forecast period from 2015 to 2023. The sera segment would witness sluggish growth due to the risk related to the protein and viral contamination and safety hazards related to the incorporation of serum from animal source which is driving the trend of serum-free media.

    To read more please go the CC report here and to find out more about
    PELOBiotech’s leading portfolio regarding these new media, please click here.

     

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  • CellTool: Next Generation Living Cell Analysis

    In Allgemein on

    Want to know how BioRAM can help you with your research? Our partner CellTool provides you wiht this overview about the working principle of its BioRam(R) device. The Raman-Trapping-Microscope  enables label-free analysis of living cells! Only a few (60-100) cells are needed to provide reliable results. In addition, some application examples are listed.For all interested people, who’d like to know, what CellTool is all about, please follow this link (in German or English). You may also get the overview and follow the CSO, Dr Karin Schütze,  on LinkedIn.

     

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  • Long-Term Outcome of Patients with Perimembranous Ventricular Septal Defect

    In Allgemein on

    Isolated ventricular septal defect (VSD) is the most common congenital heart defect, apart from bicuspid aortic valve, accounting for 30-40% of all congenital cardiac malformations. Spontaneous closure in the first years of life is common in cases of small defects. VSD is generally diagnosed and, if necessary, treated in childhood. Four subgroups have been defined in the guidelines, according to defect location: perimembranous, muscular, outlet and inlet type. The perimembranous type is the most common in adults (about 80% of all VSDs). The clinical presentation and natural history can vary from small VSD with insignificant left-to-right shunt to VSD with significant left-to-right shunt with left ventricular (LV) volume overload and right ventricular (RV) pressure overload, which, if unrepaired, may cause pulmonary vascular disease and even Eisenmenger syndrome. Patients with a small VSD and insignificant left-to-right shunt or with a repaired VSD usually remain event-free during follow-up. However, several problems may still develop later in life, with the most important being endocarditis, LV dilatation due to volume overload, double-chambered right ventricle, LV outflow tract obstruction (LVOTO), aortic valve regurgitation (AR) and complete heart block (especially in the earlier years of cardiac surgery).

    To read more please click here.

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  • Amyloid Deposits Not Always Toxic to Cells

    In Allgemein on

    How do protein aggregates damage cells? Scientists believe they are toxic themselves, or that they sequester other proteins that have an essential cellular function. In the November Science, researchers led by Frederic Rousseau and Joost Schymkowitz at VIB Switch Laboratory, Leuven, Belgium, add evidence for the latter. They designed a synthetic peptide consisting of an amyloidogenic fragment of vascular endothelial growth factor receptor 2 (VEGFR2). When added to endothelial cell cultures, which depend on VEGF signaling, the fragment triggered clumping of the receptor, and the cells died. When added to cells which express transgenic VEGFR2 but which do not normally make this receptor, the peptide seeded VEGFR2 aggregation, but the cells appeared normal. The findings suggest that at least in this case, toxicity occurs only through depriving cells of an essential protein.
    To read more please click here.

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  • Researchers describe Bone Marrow SC Population with Potential for repeat Transplantation

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    A new study demonstrates that non-blood cell forming stem cells present in human bone marrow play an important role in maintaining the hematopoietic microenvironment, and these stromal cells appear to retain full self-renewal potential after primary and secondary transplantations, according to an article published in Stem Cells and Development.
    To read more, please click here.

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  • Demand fuels 3D CC Market

    In 3D Cell Culture, Allgemein, Cell Culture on

    The global 3D cell culture market is anticipated to reach USD 1.69 billion by 2024, according to a new report by Grand View Research, Inc. Potential of this technology to replace and reduce the usage of animal models for histological analysis and biochemical assays is expected to fuel demand for 3D cell culture products thus driving growth in the coming years.
    To read more, please click here.

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  • StemCells Provide in vitro Models for Deafness

    In Allgemein, Stem Cell Culture, Stem Cells on

    A collaboration, including researchers from Juntendo University, demonstrates differentiation from stem cells into specialised cells thought to be the most important therapeutic target for the treatment of hereditary deafness.
    One in 1000 children suffers deafness or hearing loss, and hearing is the most common sense to be affected by congenital disease. Deafness at birth is often caused by mutations in a specific gene known as Gap Junction Beta 2 (GJB2), which codes for the protein connexin 26. In some populations, mutations of this gene are responsible for as many as half the instances of congenital hearing loss. Now, Kazusaku Kamiya and the co-authors of a recent report demonstrate a means of producing supplies of these cells on demand for use in therapeutic studies.

    To read more please click here.

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  • Munich Team won iGEM Award in US with 3D Printer

    In 3D Cell Culture, Allgemein on

    Congratulations to the Munich TU and LMU team: They won in Boston, US, the Bioengeeneering award iGEM in the category „Overgraduate“ . More here in German:

    Das Doppelteam von TU München und Ludwig-Maximilians-Universität (LMU) konnte mit einem 3D-Zelldrucker und passender Bio-Tinte die Fachjury am deutlichsten überzeugen. Mehr als 300 Hochschulteams aus aller Welt hatten beim „Giant Jamboree“ ihre Projekte zur Synthetischen Biologie präsentiert. Die deutschen Teams kehren mit reichlich Medaillen und Titeln von dem Megaevent an der US-Ostküste zurück: Für die 13 hiesigen Teams gab es fünfmal Gold, sechsmal Silber und zweimal Bronze.

    More here in German:

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  • Embryonic SCs and Fetal Tissue Research— will Trump Intervene?

    In Allgemein, Cell Culture on

    Of all the materials valued in biomedical research, embryonic stem (ES) cells and fetal tissue have gotten disproportionate attention from politicians. Because creating ES cell lines initially requires destroying a human embryo, President George W. Bush tightly restricted the use of federal funds for research on all but a few stem cell lines. President Barack Obama then made lifting those restrictions one of his first official actions after he took office in 2009.

    More recently, accusations that abortion clinics were unlawfully selling fetal tissue to researchers has stoked opposition to that type of research. So far, however, members of Congress have been unable to enact any restrictions into law.

    Now, biomedical researchers are wondering: How will a Donald J. Trump administration handle these ethically delicate materials?

    To read more about the concerns, please click here.

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  • New Product: Human iPSC-Derived Neural Stem Cells (i-HNSC)

    In Allgemein, product news on

    Human iPSC-Derived Neural Stem Cells (i-HNSC) are homogeneous, self-renewing and multipotent. Through partnership with leading stem cell company StemoniX, the cells are produced using next generation manufacturing technology. The result is large quantities of quality cells produced from each donor in a short period of time, cost effectively.

    Excellent Cell-Based Research Model

    • Neural Development
    • Neurotoxicity
    • Lineage Differentiation
    • Electrophysiology
    • Disease modeling
    • Drug discovery & development

    To learn more about this new product by our partner CellApplications  just give us a call or write an email to info@pelobiotech.com

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  • Wash your cloths, now: Dirty Laundry may cause Environmental Contamination

    In Allgemein on

    A new paper published in FEMS Microbiology Letters, resulting from an investigation of a laundry facility that services several Seattle-area hospitals, suggests that soiled clinical linens may be a source of surface Clostridium difficile contamination.

    C. difficile is a hospital and community acquired pathogen. C. difficile are spore-forming anaerobic bacteria that have been identified in 2-3% of healthy, non-hospitalized adults and in 10-25% of hospitalized adults. Toxin-producing C. difficile is the most common cause of hospital-acquired diarrhea. It is estimated that 25% of all C. difficile infections occur from exposures in the community that may stem from potential sources including water, soil, livestock, meats, vegetables and pets. There is evidence that C. difficile infections are seasonal and are correlated with 151 respiratory illnesses in the winter, due to antibiotic use.

    To read more, please clik here.

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  • Deep Insights: Let´s have a Look at the Marihuana Receptor

    In Allgemein on

    Crystal Structure of the Human Cannabinoid Receptor CB1

    Cannabinoid receptor 1 (CB1) is the principal target of Δ9-tetrahydrocannabinol (THC), a psychoactive chemical from Cannabis sativa with a wide range of therapeutic applications and a long history of recreational use. CB1 is activated by endocannabinoids and is a promising therapeutic target for pain management, inflammation, obesity, and substance abuse disorders. Here, we present the 2.8 Å crystal structure of human CB1 in complex with AM6538, a stabilizing antagonist, synthesized and characterized for this structural study. The structure of the CB1-AM6538 complex reveals key features of the receptor and critical interactions for antagonist binding. In combination with functional studies and molecular modeling, the structure provides insight into the binding mode of naturally occurring CB1 ligands, such as THC, and synthetic cannabinoids. This enhances our understanding of the molecular basis for the physiological functions of CB1 and provides new opportunities for the design of next-generation CB1-targeting pharmaceuticals.
    To read full article, please click here.

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  • FREE Webinar: Marvelous MSCs

    In Allgemein, Webinar on

    Marvelous MSCs: Tipps, Tricks and Tools for your MSC Cultures

    How MSC sourcing interact with your project.
    presented by Peter Frost , PhD, PELOBIOTECH, Germany
    Date: Th, Oct 25, 2016,
    Time: 10:00 AM or 3:00 PM (CET), language: German and English

    Mesenchymal stem cells (MSCs) are defined as multipotent stem cells being able for adipogenic, chondrogenic and osteogenic differentiation. Furthermore MSCs have to be positive for CD90, CD105 and CD73, and lacking the expression of CD14, CD34 and CD45.

    To sign up, please click here.

     

    Please share this information with your colleagues and friends.

    Thank you.

    Yopur PELOBIOTECH-Team

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