• New: Reprogramming Kit for five mRNA Factors

    In Allgemein, product news, Stem Cell Culture on

    The Stemgent #mRNA Reprogramming Kit, in combination with the microRNA Booster Kit (00-0073), is the fastest, safest, and most efficient method for generating integration-free, virus-free, clinically relevant human #iPS cells. Generate iPS cells in less than 16 days and expand new lines in culture in as little as 3 weeks as compared to lentivirus, Sendai virus, and other methods, that can take over 20 weeks to confirm that viral remnants no longer remain. This system completely eliminates the concern of virus bio-containment and safety issues.
    Fully tested and validated, the Stemgent mRNA Reprogramming Kit includes five mRNA reprogramming factors, a nuclear GF P marker for monitoring transfection efficiency, the Stemgent Pluriton™ Reprogramming Medium, B18R protein, and a reproducible, optimized protocol. This kit has been validated to successfully reprogram human BJ fibroblasts as a control cell line. The Stemgent mRNA Reprogramming Kit enables researchers greater ease-of-use with the ability to simply regulate the timing of protein expression levels and have stoichiometric control of individual reprogramming factors.

    Advantages
    • Non-integrating — no risk of genomic integration
    • Faster reprogramming, colonies can emerge appear in as early as 12 days
    • Stoichiometric control of individual reprogramming factors
    • Eliminates need for multi-step passaging and screening of colonies
    • Validated protocol using normal and diseased cells
    • Hands-on training and on-site support

    For more information, click here give us a call or send an email to info@pelobiotech.com

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  • FREE Webinar BioRam: New Generation of Cell Analysing

    Sign up for this FREE Webinar now.

    #BioRam: New Generation of Cell Analysing

    presented by
    Karin Schütze, PhD, CSO CellTool, Germany, and
    Heidi Kremling, PhD, Scientific Head of Lab, CellTool

    Part 1, 10 AM: BioRam® Raman-Trapping-Microscopy, next generation single cell analysis and cell sorting

    Part 2, 10:30 AM: BioRam® Raman-Trapping-Microscopy analyzing #3D-tissues and Spheroids
    Date: Tue, 24 January 2017
    Time: 10 AM (CET)
    Sign up here now.

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  • How Human Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) interact

    In Allgemein on

    #Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) is a major component of the endothelial cell intercellular junction. Previous studies have shown that PECAM-1 homophilic interactions, mediated by amino-terminal immunoglobulin homology domain 1, contribute to maintenance of the vascular permeability barrier and to its re-establishment following inflammatory or thrombotic insult. PECAM-1 glycans account for ∼30% of its molecular mass, and the newly solved crystal structure of human PECAM-1 immunoglobulin homology domain 1 reveals that a glycan emanating from the asparagine residue at position 25 (Asn-25) is located within the trans homophilic-binding interface, suggesting a role for an Asn-25-associated glycan in PECAM-1 homophilic interactions. In support of this possibility, unbiased molecular docking studies revealed that negatively charged α2,3 sialic acid moieties bind tightly to a groove within the PECAM-1 homophilic interface in an orientation that favors the formation of an electrostatic bridge with positively charged Lys-89, mutation of which has been shown previously to disrupt PECAM-1-mediated homophilic binding.

    To read more follow this link.

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  • See Where the 3D and Cell Culture Market is Heading to

    In 3D Cell Culture, Allgemein, Cell Culture on

    The Global Cell Culture Market is poised to grow at a CAGR of around 11.4% over the next decade to reach approximately $33.47 billion by 2025. Some of the prominent trends that the market is witnessing include 3D cell culture will offer a significant opportunity in this market, increased risk of communicable diseases and pandemics, increasing preference for single use technologies and recent technological developments of cell culture.

    Based on product, the market is categorized into consumables and equipment. By consumable, the market is further segmented into reagents, sera and media. By equipment, the cell culture market is further divided into cell culture supporting equipment, cell culture vessels, bioreactors and cell culture storage equipment.

    Depending on the end user, the market is segmented into pharmaceutical and biotechnology companies and research institutes.

    To read more please click here

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  • How the new US President will influence the Cell Culture Branch

    In Allgemein on

    President-elect Trump’s cabinet appointments are generating a great deal of attention and controversy. After eight years of Democrat rule, it would be foolish to expect otherwise.

    One appointment, though, will have a profound impact on your health… and the health of your loved ones.

    The “progressive approval” process for drugs

    The first potential FDA chief appointee leaked by the Trump transition team is Jim O’Neill. Managing director of Peter Thiel’s Mithril Capital, O’Neill has publicly supported proposals to do away with the FDA’s requirement for phase 2 and 3 trials. Instead, he favors “progressive approval” of drugs and other medical technologies.

    Read the full Forbes-article here.

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  • Mitomycin C-treated human-induced Pluripotent Stem Cells as a Safe delivery System of Gold Nanorods

    In Allgemein, Stem Cell Culture on

    Human-induced pluripotent stem cells (#iPS) possess an intrinsic tumor tropism ability. However, iPS cells are impeded in clinical applications of tumor therapy due to the formation of teratomas and their survival in normal organs such as the liver, lungs, spleen and kidneys. Mitomycin C (#MMC) can overcome this limitation by suppressing iPS proliferation. Herein, we fabricated a safe delivery system of iPS cells treated with MMC loading with gold nanorods (AuNRs) for the targeted photothermal treatment of #gastric cancer. Our results showed that the tumor cells were efficiently killed by the heat generated from the gold nanorods, and the iPS cells ultimately died due to the action of MMC seven days after the photothermal treatment. This suggested that pre-treated iPS cells with MMC can be used as a novel and safe approach for targeted tumor therapy. This paves the road for clinical translation in the future.

    To read more please click here.

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  • Kinetics of circulating EPCs

    In Allgemein on

    Endothelial progenitor cells (EPCs) are primitive cells found in the bone marrow and peripheral blood (PB). In particular, the potential of EPCs to differentiate into mature endothelial cells remains of high interest for clinical applications such as bio-functionalized patches for autologous seeding after implantation. The objective of this study was to determine EPCs’ kinetics in patients undergoing carotid artery thromboendarterectomy (CTEA) and patch angioplasty.
    Methods: Twenty CTEA patients were included (15 male, mean age 76 years). PB samples were taken at 1 day preoperatively, and at 1, 3, and 5 days postoperatively. Flow cytometric analysis was performed for CD34, CD133, KDR, and CD45. Expression of KDR, SDF-1α, and G-CSF was analyzed by means of enzyme-linked immunosorbent assay.
    Results: Fluorescence-activated cell sorting analysis revealed 0.031%±0.016% (% of PB mononuclear cells) KDR+ cells and 0.052%±0.022% CD45-/CD34+/CD133+ cells, preoperatively. A 33% decrease of CD45–/CD34+/CD133+ cells was observed at day 1 after surgery. However, a relative number (compared to initial preoperative values) of CD45-/CD34+/CD133+ cells was found on day 3 (82%) and on day 5 (94%) postoperatively. More profound upregulated levels of CD45–CD34+/CD133+ cells were observed for diabetic (+47% compared to nondiabetic) and male (+38% compared to female) patients. No significant postoperative time-dependent differences were found in numbers of KDR+ cells and the concentrations of the cytokines KDR and G-CSF. However, the SDF-1α levels decreased significantly on day 1 postoperatively but returned to preoperative levels by day 3.
    Conclusion: CTEA results in short-term downregulation of circulating EPCs and SDF-1α levels. Rapid return to baseline levels might indicate participation of EPCs in repair mechanisms following vascular injury.

    To read the full article please click here.

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  • Astrocytes and Exosomes Implicated in Protein Propagation

    In Allgemein on

    Many researchers now believe that misfolded proteins spread from cell to cell across the brain, corrupting normal proteins as they go, yet exactly how this propagation would happen remains unclear. At the Society for Neuroscience annual meeting  scientists identified specific mechanisms that may be involved in the spread of pathological proteins in Parkinson’s and Alzheimer’s diseases. They argued that astrocytes transmit α-synuclein aggregates by cell-to-cell contact; for tau, they argued that its trans-synaptic spread depends on the secretory vesicles known as exosomes. In addition, researchers debuted a new method for following tau aggregates over time in living mouse brain.

    “Up until a few years ago, we did not understand how Parkinson’s disease progresses at the molecular level,” noted Alice Chen-Plotkin of the University of Pennsylvania. “Now, we may be starting to discover how the disease worsens, which could give insights into ways to slow it down or stop it,” she wrote to Alzforum.

    To read more, please click here.

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  • New clues to Myelination could help Identify ways to Intervene in Neurodegenerative Diseases

    In Allgemein on
    “If we can further enhance our understanding of how these oligodendrocyte precursor cells mature, then it may be possible to stimulate them to replace myelin in diseases like multiple sclerosis.”, says Pablo Paez, PhD, Assistant professor and research scientist , unters James Kelly Research Institute at University at Buffalo, US.

     The Researchers at the University at Buffalo have identified a critical step in myelination after birth that has significance for treating neurodegenerative diseases like multiple sclerosis, in which myelin is lost or damaged. Myelin is the protective coating that neurons need to function. The preclinical research  concerns oligodendrocytes, the cells that make myelin, and the progenitor cells that are their precursors. The work involved the study of voltage-operated calcium channels, which are responsible for initiating many physiological functions.

    To read more how a certain type of calcium channel is critical and may lead to improved therapies for diseases like multiple sclerosis please click here.

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  • Biopreservation Market is getting More and More Important

    In Allgemein, regenerative medicine on

    Increasing demand for preserving stem cells and rise in the number of egg & sperm banks along with the growing implementation of assisted reproductive technology (ART) across the globe is expected to boost the Biopreservation market demand over the coming years. Growing healthcare awareness among the people as well as technological breakthroughs in healthcare are predicted to complement the industry growth.

    A large global population suffering from lifestyle related diseases as well as cardiovascular disorders, high blood pressure, and cancer is predicted to promote the expansion of the market over the coming eight years. The increasing popularity of regenerative drugs along with wide biobanking application for cell, organ, and tissue preservation is further predicted to benefit the industry in near future. According to Global Market Insights Inc., “The biopreservation market size worth USD 3 billion in 2015, is predicted to exceed USD 9.7 billion mark, growing at a CAGR of 13.4% over the period of 2016-2024.” To read more, please click here.

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  • For Stem Cells to Be Healthy, Telomere Length Has to Be Just Right

    In Allgemein, regenerative medicine, Stem Cells on

    Ever since researchers connected the shortening of telomeres–the protective structures on the ends of chromosomes–to ageing and disease, the race has been on to understand the factors that govern telomere length.

    Now, scientists at the Salk Institute have found that a balance of elongation and trimming in stem cells results in telomeres that are, as Goldilocks would say, not too short and not too long, but just right.

    The finding, which appears in the December, 2016, issue of Nature Structural & Molecular Biology, deepens our understanding of stem cell biology and could help advance stem cell-based therapies, especially related to ageing and regenerative medicine.

    To read more please click here.

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  • Cell Signalling in the Cardiovascular System

    In Allgemein on

    The cardiovascular system is a highly complex, well organised system in which signal transduction plays critical physiological and pathophysiological roles. The cellular elements of the heart and vascular wall are equipped with an array of specific receptors and with complex intracellular machinery that facilitates and drives appropriate responses to extracellular stimuli. Understanding the mechanisms through which extracellular stimuli modify the functions of cells in the heart and vascular wall gives valuable insights into how perturbations of signalling systems can cause pathological situations. This knowledge will allow the identification of novel molecular targets for pharmacological intervention and will assist the future development of therapeutic strategies for managing cardiovascular disorders. This brief review will give a general overview of some major intracellular signalling systems operative in cells comprising the heart and vasculature, with particular emphasis on the pleiotropic roles of protein kinases as regulators of cell behaviour.

    To read this overview, please click here.

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  • Gut Tissue with Functional Nervous System Created in Laboratory for First Time

    In Allgemein, iPS media, regenerative medicine on

    Scientists created human gut tissue containing a functional nervous system in the laboratory for the first time.

    According to the authors of the study, “Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system,” which was published in the leading scientific journal Nature Medicine, this research could help treat people with conditions such as inflammatory bowel disease (IBD). However, they warned: “The complexity of the [gut] tissue will demand much more work before it reaches prime time in patients.”

    The team of researchers, led by James Wells, PhD, of the Division of Developmental Biology at Cincinnati Children’s Hospital Medical Center in Ohio, USA used a tissue engineering approach with embryonic stem (ES) cells and another type of stem cells called induced pluripotent stem cells (IPS) derived from either skin or white blood cells, to generate the human gut tissue.

    Read more here or the official statement.
    You can also watch the following YouTube here.

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  • Defined and Serum-free Media on the Rise

    In Allgemein on

    The global cell culture market has been broadly segmented into media, sera, and reagents. Chemically-defined media rarely provide desired yield, and thus the inclination toward more complex media increases. The adoption of chemically-defined media has been slow yet steady for both media manufacturers and bioprocessors. Demand for media has been shifting from serum-based to serum-sparing, serum-free animal derived, and chemically-defined media. Lysogeny broth (LB) is a nutritive medium used for artificial bacteria growth.

    The attachment factors segment accounted for the largest share of more than 25% of the global cell culture reagents market in 2014. However, amino acids is estimated to be the fastest growing segment during the forecast period from 2015 to 2023. The sera segment would witness sluggish growth due to the risk related to the protein and viral contamination and safety hazards related to the incorporation of serum from animal source which is driving the trend of serum-free media.

    To read more please go the CC report here and to find out more about
    PELOBiotech’s leading portfolio regarding these new media, please click here.

     

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  • CellTool: Next Generation Living Cell Analysis

    In Allgemein on

    Want to know how BioRAM can help you with your research? Our partner CellTool provides you wiht this overview about the working principle of its BioRam(R) device. The Raman-Trapping-Microscope  enables label-free analysis of living cells! Only a few (60-100) cells are needed to provide reliable results. In addition, some application examples are listed.For all interested people, who’d like to know, what CellTool is all about, please follow this link (in German or English). You may also get the overview and follow the CSO, Dr Karin Schütze,  on LinkedIn.

     

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  • Long-Term Outcome of Patients with Perimembranous Ventricular Septal Defect

    In Allgemein on

    Isolated ventricular septal defect (VSD) is the most common congenital heart defect, apart from bicuspid aortic valve, accounting for 30-40% of all congenital cardiac malformations. Spontaneous closure in the first years of life is common in cases of small defects. VSD is generally diagnosed and, if necessary, treated in childhood. Four subgroups have been defined in the guidelines, according to defect location: perimembranous, muscular, outlet and inlet type. The perimembranous type is the most common in adults (about 80% of all VSDs). The clinical presentation and natural history can vary from small VSD with insignificant left-to-right shunt to VSD with significant left-to-right shunt with left ventricular (LV) volume overload and right ventricular (RV) pressure overload, which, if unrepaired, may cause pulmonary vascular disease and even Eisenmenger syndrome. Patients with a small VSD and insignificant left-to-right shunt or with a repaired VSD usually remain event-free during follow-up. However, several problems may still develop later in life, with the most important being endocarditis, LV dilatation due to volume overload, double-chambered right ventricle, LV outflow tract obstruction (LVOTO), aortic valve regurgitation (AR) and complete heart block (especially in the earlier years of cardiac surgery).

    To read more please click here.

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  • Amyloid Deposits Not Always Toxic to Cells

    In Allgemein on

    How do protein aggregates damage cells? Scientists believe they are toxic themselves, or that they sequester other proteins that have an essential cellular function. In the November Science, researchers led by Frederic Rousseau and Joost Schymkowitz at VIB Switch Laboratory, Leuven, Belgium, add evidence for the latter. They designed a synthetic peptide consisting of an amyloidogenic fragment of vascular endothelial growth factor receptor 2 (VEGFR2). When added to endothelial cell cultures, which depend on VEGF signaling, the fragment triggered clumping of the receptor, and the cells died. When added to cells which express transgenic VEGFR2 but which do not normally make this receptor, the peptide seeded VEGFR2 aggregation, but the cells appeared normal. The findings suggest that at least in this case, toxicity occurs only through depriving cells of an essential protein.
    To read more please click here.

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  • Researchers describe Bone Marrow SC Population with Potential for repeat Transplantation

    In Allgemein on

    A new study demonstrates that non-blood cell forming stem cells present in human bone marrow play an important role in maintaining the hematopoietic microenvironment, and these stromal cells appear to retain full self-renewal potential after primary and secondary transplantations, according to an article published in Stem Cells and Development.
    To read more, please click here.

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  • Demand fuels 3D CC Market

    In 3D Cell Culture, Allgemein, Cell Culture on

    The global 3D cell culture market is anticipated to reach USD 1.69 billion by 2024, according to a new report by Grand View Research, Inc. Potential of this technology to replace and reduce the usage of animal models for histological analysis and biochemical assays is expected to fuel demand for 3D cell culture products thus driving growth in the coming years.
    To read more, please click here.

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  • StemCells Provide in vitro Models for Deafness

    In Allgemein, Stem Cell Culture, Stem Cells on

    A collaboration, including researchers from Juntendo University, demonstrates differentiation from stem cells into specialised cells thought to be the most important therapeutic target for the treatment of hereditary deafness.
    One in 1000 children suffers deafness or hearing loss, and hearing is the most common sense to be affected by congenital disease. Deafness at birth is often caused by mutations in a specific gene known as Gap Junction Beta 2 (GJB2), which codes for the protein connexin 26. In some populations, mutations of this gene are responsible for as many as half the instances of congenital hearing loss. Now, Kazusaku Kamiya and the co-authors of a recent report demonstrate a means of producing supplies of these cells on demand for use in therapeutic studies.

    To read more please click here.

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