• ReSyn Biosciences participating as Industrial Partner in Horizon 2020 BioCapture Project

    In Allgemein on

    We congratuate our partner ReSyn to be part of a Horizon 2020 project. BioCapture is a European Training Network (ETN) funded by the European Commission under the #Horizon 2020 Marie Skłodowska-Curie Action. The network consists of 15 research groups, spread across 11 universities and institutes and 4 industrial partners in 6 different countries that have come together to train a new generation of chemists/physicists/biologists through an EU-wide PhD training network. The main aim of this interdisciplinary project is to discover new and improved ways to diagnose and treat cancer.

    BioCapture offers an opportunity for 11 early stage researchers (ESR’s) to develop molecularly imprinted polymers (MIP’s), also referred to as “plastic antibodies”, for specific application to the discovery and detection of cancer #biomarkers.
    Read more here or at Linked-In.

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  • 3D Tissue Culture Model Mimics Gut Infections

    In Allgemein on

    With threats like zika, ebola, #SARS and tuberculosis increasing, researchers are using predictive tissue culture models to more accurately reflect how humans respond to pathogens.

    The lack of sophisticated models has greatly slowed the ability to understand infectious disease from both the host and the pathogen perspective.

    One approach to address this problem is the use of predictive tissue culture models. To develop these models, an understanding of how cells and tissues in our bodies function in a #3D context is required.

    A research team from the Arizona State University Biodesign Institute team and NASA’s Johnson Space Center, have developed a realistic 3D co-culture model, which incorporates an important immune defense cell type found in the intestine, macrophages, key cells targeted by salmonella, a leading cause of food poisoning and systemic disease worldwide.

    Read more here.

     

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  • NEWS: Meet us at…

    In 3D Cell Culture, Allgemein on

    ELRIG Forum 2017 in Darmstadt, Germany.
    This interesting meeting in the Wissenschafts- und Kongresszentrum “darmstadtium” starts today, March 9, 2017 any minute.

    Topic: Phenotypic Screening und #3D Cellular Screening Models
    So come along and meet PELOBIOTECH at #Elrig Forum, Booth 28, and discuss with Dr. Peter Frost, CEO, and other scientists the latest approaches of academic and industrial research.

    More information here.

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  • SC treatment may Restore Vision

    In Allgemein, Stem Cell Culture on

    Researchers working as part of the University of Georgia’s Regenerative Bioscience Center have developed a new way to identify and sort #stem cells that may one day allow clinicians to restore vision to people with damaged corneas using the patient’s own eye tissue. They published their findings in Biophysical Journal.

    The #cornea is a transparent layer of tissue covering the front of the eye, and its health is maintained by a group of cells called limbal stem cells. But when these cells are damaged by trauma or disease, the cornea loses its ability to self-repair.

    Raed more here.

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  • Quit smoking? Cigarette smoke blocks self-healing processes

    In Allgemein on

    Smoke from cigarettes blocks self-healing processes in the lungs and consequently can lead to chronic obstructive #pulmonary disease (COPD), says a new study. Researchers at the #Helmholtz Zentrum München, partner in the German Centre for Lung Research (DZL), and their international colleagues have reported this finding in the American Journal of Respiratory and Critical Care Medicine.

    Cough, bronchitis and breathing difficulties are the typical manifestations of COPD. Exact figures are not available, but estimates assume that 10 to 12% of adults over 40 years of age in Germany suffer from the disease. Experts estimate the national economic costs of the disease at almost six billion euros annually. Scientists around the world are attempting to discover how the disease develops and what biological adjustments can be made to stop it.

    To read more click here please.

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  • Scientists rejuvenate Blood by Cell Reprogramming

    In Allgemein on

    When we are young, our blood #stem cells produce an even and well-balanced number of red and white blood cells according to need. As we age, however, the capacity of the blood stem cells to produce the number of blood cells we need declines. Like it or not, your body gradually loses its capacity for producing blood as you age — that’s why immune deficiencies, leukemia and other blood conditions become more likely with time. Scientists may have found a way to turn back the clock, though. They’ve found a way to rejuvenate blood by reprogramming the stem cells that create it. The team effectively “resets” the stem cells by turning them into #iPS cells, which can generate any kind of cell. When they once again form blood stem cells, it’s as if they were brand new.

    “This type of age-related change can have major consequences as it can lead to an imbalance in stem cell production. For example, a reduced production of immune cells or excessive production of other types of cells can be a precursor to leukaemia”, explains David Bryder, who headed the study at Lund University.

    Read more here and here.

     

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  • How Cerebral Malaria Attacks the Brain

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    Most clinicians know that cerebral #malaria, a complication associated with the all-too-common mosquito-borne virus that affects much of the developing world, can be deadly; however, relatively little is known about exactly how the disease attacks the human #brain.

    That may be changing. Using mouse models the authors of a study demonstrated that cytotoxic #T-cells attack cerebral blood vessels leading to swelling in the brain. They believe that their findings mirror how cerebral malaria affects the human brain. The complication is associated with Plasmodium falciparum malaria, the most dangerous form of the virus. Exact figures on its incidence are not available; however, it is believed that more than 500,000 children develop cerebral malaria annually in Africa alone, and that it causes as many as 1 million deaths worldwide each year. It is fatal in as many as 30% of the people it afflicts. Sufferers typically experience seizures and may lapse into a coma.

    See more here.

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  • Mitomycin C-treated iPS cells as a Safe Delivery System

    In Allgemein, iPS media, Stem Cell Culture on

    Human-induced pluripotent stem cells (iPS) possess an intrinsic tumor tropism ability. However, #iPS cells are impeded in clinical applications of tumor therapy due to the formation of teratomas and their survival in normal organs such as the liver, lungs, spleen and kidneys. #Mitomycin C (MMC) can overcome this limitation by suppressing iPS proliferation. Herein, we fabricated a safe delivery system of iPS cells treated with MMC loading with gold nanorods (AuNRs) for the targeted photothermal treatment of gastric cancer. Our results showed that the tumor cells were efficiently killed by the heat generated from the gold nanorods, and the iPS cells ultimately died due to the action of #MMC seven days after the photothermal treatment. This suggested that pre-treated iPS cells with MMC can be used as a novel and safe approach for targeted tumor therapy. This paves the road for clinical translation in the future.

    To read more please click here

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  • FREE Webinar: MSCs and the challenges of Xeno-free Media

    In Allgemein on

    Sign up now!
    Tue, Feb 21, 2017 10:00 AM – 11:00 PM CET

    presented by Peter Frost, PhD, CEO, PELOBIOTECH

    How the right media effects your MSC culture

    We will focus in this webinar on the

    – easy culture of MSC from different tissues
    -Xeno-free culture systems
    – best Cells, Media and Reagents for MSCs
    – Overview, what works best and what challenges do we face now and in the future
    – why it is so important to find and define the right conditions.

    Please share this information with your co-workers and friends. Thank you.

    Your PELOBiotech-Team

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  • #Regenerative Medicine Has a Bright Future

    In Allgemein, regenerative medicine on

    The Army is already using regenerative medicine to help wounded soldiers while #Duke University is researching it to help repair spinal cord injuries.

    U.S. Army scientists, working with medical technology companies, have successfully tested and used products and techniques that have enabled Army surgeons to replace the severely burned skin of soldiers as well as transplant new hands and even faces.

    At Duke University, researchers are studying zebra fish to learn how science and medicine might someday be able to regenerate severed human spinal cords.

    These examples — one already in practice and the other in the early research stages — illustrate the potential that regenerative medicine offers for the future of medical care.

    This research aims to go beyond easing the pain of life-threatening illnesses by changing the way diseases affect the body and then eradicating them.
    To read full article, click here.

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  • Cold Chain Logistics: Innovation in Biopreservation of Cell Therapies and Tissues

    In Allgemein on

    Cold chain logistics can determine success or failure for any biotech company shipping time and/or temperature-sensitive products, many of which have the potential to save lives. These include traditional solid #organs intended for transplantation into a recipient, and also a new and growing class of novel cellular therapies and engineered tissue products.

    In the high-profile and promising personalized medicine arena, a circular process is used with cold chain logistics playing a critical part in two steps. For autologous cell therapies using chimeric antigen receptor and other types of #T cells targeting various cancers, the process involves removing source material such as bone marrow, peripheral blood, or adipose tissue from a patient, shipping these to a processing and manufacturing facility, manufacturing a dose of therapeutic cells, and then shipping the cells back to the original clinical site for reinfusion into the same patient. Due to their fragile nature and limited shelf life outside the body, cold chain technologies are used to maintain a reduced temperature of these biologic materials, to slow or stop metabolic activity during transport.

    To read more, please click here

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  • NEW PELOBiotech News 02/17

    In Allgemein on

    PELONEWS 02/2017
    Dear scientist and colleague,

    we have our new Productlist for #Human Primary Cells, #SC and Media available. Just pick your favorite.
    Also there are more and more projects going on regarding MSCs. That´s why we get up close in our next FREE webinar mid Feburary. Just sign up and we send the record to you afterwards – even if you have no time on Tuesdays.

    To get more, plcase click here

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  • Shape of Tumor may affect whether Cells can Metastasize

    In Allgemein, Stem Cells on

    Only a few cells in a cancerous tumor are able to break away and spread to other parts of the body, but the curve along the edge of the tumor may play a large role in activating these tumor-seeding cells, according to a new University of Illinois study.

    Using engineered tissue environments in various shapes and patterns, the study of skin cancer found that the more curved the cell cultures were, the more cancer cells at the edges displayed markers o f#stem cell characteristics – the key to spreading to other tissues.

    This has potential for furthering our understanding of cancer as well as developing personalized treatment plans.

    Led by Kristopher Kilian, a professor of materials science and engineering, and Timothy Fan, a professor of veterinary medicine, the researchers published their findings in the journal Nature Materials.

    “The most dangerous part of cancer is metastasis,” Kilian said. “Some cells that we call #cancer stem cells adopt deadly characteristics where they can travel through the bloodstream to other tissue and form new tumors.”

    To read more, please click here

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  • ‘Totipotent’ SC capable of forming Viable Embryo

    In Allgemein, Cell Culture, Stem Cell Culture on

    #Stem cells with characteristics of totipotency — capable of creating all the tissue types needed to build and sustain an embryo — have been created in mice by a California-led research team.

    Researchers found that a #microRNA called miR-34a prevents totipotency. Pluripotent mouse cells deficient in that microRNA acquired signs of totipotency.

    The feat makes it feasible to explore the molecular basis of totipotency, said the researchers in a study, that was led by Yong Jin Choi and Chao-Po Lin of UC Berkeley. Lin He, also of UC Berkeley, was the senior author. It can be found at j.mp/totipot.

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  • New: Reprogramming Kit for five mRNA Factors

    In Allgemein, product news, Stem Cell Culture on

    The Stemgent #mRNA Reprogramming Kit, in combination with the microRNA Booster Kit (00-0073), is the fastest, safest, and most efficient method for generating integration-free, virus-free, clinically relevant human #iPS cells. Generate iPS cells in less than 16 days and expand new lines in culture in as little as 3 weeks as compared to lentivirus, Sendai virus, and other methods, that can take over 20 weeks to confirm that viral remnants no longer remain. This system completely eliminates the concern of virus bio-containment and safety issues.
    Fully tested and validated, the Stemgent mRNA Reprogramming Kit includes five mRNA reprogramming factors, a nuclear GF P marker for monitoring transfection efficiency, the Stemgent Pluriton™ Reprogramming Medium, B18R protein, and a reproducible, optimized protocol. This kit has been validated to successfully reprogram human BJ fibroblasts as a control cell line. The Stemgent mRNA Reprogramming Kit enables researchers greater ease-of-use with the ability to simply regulate the timing of protein expression levels and have stoichiometric control of individual reprogramming factors.

    Advantages
    • Non-integrating — no risk of genomic integration
    • Faster reprogramming, colonies can emerge appear in as early as 12 days
    • Stoichiometric control of individual reprogramming factors
    • Eliminates need for multi-step passaging and screening of colonies
    • Validated protocol using normal and diseased cells
    • Hands-on training and on-site support

    For more information, click here give us a call or send an email to info@pelobiotech.com

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  • FREE Webinar BioRam: New Generation of Cell Analysing

    Sign up for this FREE Webinar now.

    #BioRam: New Generation of Cell Analysing

    presented by
    Karin Schütze, PhD, CSO CellTool, Germany, and
    Heidi Kremling, PhD, Scientific Head of Lab, CellTool

    Part 1, 10 AM: BioRam® Raman-Trapping-Microscopy, next generation single cell analysis and cell sorting

    Part 2, 10:30 AM: BioRam® Raman-Trapping-Microscopy analyzing #3D-tissues and Spheroids
    Date: Tue, 24 January 2017
    Time: 10 AM (CET)
    Sign up here now.

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  • How Human Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) interact

    In Allgemein on

    #Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) is a major component of the endothelial cell intercellular junction. Previous studies have shown that PECAM-1 homophilic interactions, mediated by amino-terminal immunoglobulin homology domain 1, contribute to maintenance of the vascular permeability barrier and to its re-establishment following inflammatory or thrombotic insult. PECAM-1 glycans account for ∼30% of its molecular mass, and the newly solved crystal structure of human PECAM-1 immunoglobulin homology domain 1 reveals that a glycan emanating from the asparagine residue at position 25 (Asn-25) is located within the trans homophilic-binding interface, suggesting a role for an Asn-25-associated glycan in PECAM-1 homophilic interactions. In support of this possibility, unbiased molecular docking studies revealed that negatively charged α2,3 sialic acid moieties bind tightly to a groove within the PECAM-1 homophilic interface in an orientation that favors the formation of an electrostatic bridge with positively charged Lys-89, mutation of which has been shown previously to disrupt PECAM-1-mediated homophilic binding.

    To read more follow this link.

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  • See Where the 3D and Cell Culture Market is Heading to

    In 3D Cell Culture, Allgemein, Cell Culture on

    The Global Cell Culture Market is poised to grow at a CAGR of around 11.4% over the next decade to reach approximately $33.47 billion by 2025. Some of the prominent trends that the market is witnessing include 3D cell culture will offer a significant opportunity in this market, increased risk of communicable diseases and pandemics, increasing preference for single use technologies and recent technological developments of cell culture.

    Based on product, the market is categorized into consumables and equipment. By consumable, the market is further segmented into reagents, sera and media. By equipment, the cell culture market is further divided into cell culture supporting equipment, cell culture vessels, bioreactors and cell culture storage equipment.

    Depending on the end user, the market is segmented into pharmaceutical and biotechnology companies and research institutes.

    To read more please click here

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  • How the new US President will influence the Cell Culture Branch

    In Allgemein on

    President-elect Trump’s cabinet appointments are generating a great deal of attention and controversy. After eight years of Democrat rule, it would be foolish to expect otherwise.

    One appointment, though, will have a profound impact on your health… and the health of your loved ones.

    The “progressive approval” process for drugs

    The first potential FDA chief appointee leaked by the Trump transition team is Jim O’Neill. Managing director of Peter Thiel’s Mithril Capital, O’Neill has publicly supported proposals to do away with the FDA’s requirement for phase 2 and 3 trials. Instead, he favors “progressive approval” of drugs and other medical technologies.

    Read the full Forbes-article here.

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  • Mitomycin C-treated human-induced Pluripotent Stem Cells as a Safe delivery System of Gold Nanorods

    In Allgemein, Stem Cell Culture on

    Human-induced pluripotent stem cells (#iPS) possess an intrinsic tumor tropism ability. However, iPS cells are impeded in clinical applications of tumor therapy due to the formation of teratomas and their survival in normal organs such as the liver, lungs, spleen and kidneys. Mitomycin C (#MMC) can overcome this limitation by suppressing iPS proliferation. Herein, we fabricated a safe delivery system of iPS cells treated with MMC loading with gold nanorods (AuNRs) for the targeted photothermal treatment of #gastric cancer. Our results showed that the tumor cells were efficiently killed by the heat generated from the gold nanorods, and the iPS cells ultimately died due to the action of MMC seven days after the photothermal treatment. This suggested that pre-treated iPS cells with MMC can be used as a novel and safe approach for targeted tumor therapy. This paves the road for clinical translation in the future.

    To read more please click here.

    weiter Lesen

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