MicroStem – Premier Provider for Cell Culture Microenvironment Technologies

We offer MicroStem's MicroMatrix™ technology to discover and elucidate cell culture and screening conditions for many different mammalian cell types. MicroStem technology will immobillize soluble and insoluble molecules in combination as a solid phase array. Cells adhere to these arrays for real time and end point experiments. Because the platform is a micro-array it requires minimal amounts of cells to test thousands of molecules in parallel. Our products require no liquid handling or robotic instrumentation. High content analysis and other forms of image based analysis can be used.
MicroMatrix and ChamberMatrix arrays enable drug discovery assay development directly on patient samples. Custom cell attachment and assay arrays may be custom manufactured with proteins, nucleic acids, or other molecules of interest:

  • Small molecules
  • siRNA
  • microRNA
  • Antibodies
  • Peptides
  • Secreted proteins

Applications of Technology
Along with many of the most common mammalian cell lines, the MicroMatrix™ platform has been used to experiment with rare and difficult to grow cell types, including:

  • Tissue specific stem cells
  • Primary cancer cells
  • Induced Pluripotent Stem Cells (iPSC)
  • Human Embryonic Stem Cells (hESCs)
  • Epithelial Mesenchymal Transition and Cancer

Application Notes
Cytotoxicity profiling technote download
Epithelial mesenchymal transition technote download
Brafman_2009_Integrative_Bio download

Cell Attachment & Culture Arrays

MicroMatrix extra cellular matrix (ECM) arrays enable rapid screening of 36 different combinations of extra cellular matrix proteins (ECMPs) in a single experiment, requiring only a limited population of cells. Each of the 36 ECM conditions on a MicroMatrix array consists of one to several matrix proteins, spotted onto a thin hydrogel layer. Cells will attach only to the ECM spots, with greater cell density and superior morphology being observed in the matrix conditions for which the cells have an affinity. Once identified on the MicroMatrix array the ECM conditions that support your cell's attachment, morphology and other desired outcomes, can be further investigated in a chambered slide system or ordered as an optimized solution to coat labware.

Stem Cell Differentiation Arrays

The Importance of the Extra Cellular Matrix

Microenvironments play a key role in the differentiation of stem cells. When cells differentiate in vivo they do so in a complex environment that may include extra cellular matrix proteins. Different tissues contain different combinations of these proteins, such as collagens, fibronectin, vitronectin, elastins, and more. The right extra cellular matrix can enable differentiation to desired cell types as well as increasing efficiencies of established differentiation protocols.

MicroMatrix 36 ECM Arrays enable rapid and economical screening of complex ECMs for any cell type.

Mesenchymal stem cells can be differentiated into cardiomyocytes using ECMs discovered on MicroMatrix arrays.

Mesenchymal Stem Cells can be differentiated into cardiac progenitor cells and subsequently into cardiomyocytes using ECMs discovered on MicroMatrix 36 arrays.

The Importance of the Extracellular Matrix for Stem Cell Differentiation

Webinar presented by Marie Zhang Ph.D.; MicroStem, Inc.

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phone +49(0)89517 771 40
fax +49(0)89 517 771 60

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