3D Cell Culture

3D Cell Culture Systems can be developed following different strategies. Depending on the cell or cell line to be used and the application of interest different strategies has to be selected. 3D Cell Models can be produced by using hydrogels, scaffolds, hanging drop or bioprinting techniques. We at PELOBIOTECH like to offer you a broad portfolio of different 3D Cell Culture techniques to give you the chance to select the best models for your application.

We offer:

The advantage of using hydrogels is plain to see. They can easily be adjusted with regards to elasticity and stiffness. Synthetic as well as functional hydrogels are best suited for encapsulation of cells and drugs.
Scaffold-free systems give you the ability to investigate 3D cell models without having effects of external matrix proteins, if this is suitable for your application. Scaffold-free 3D models can be produced using the hanging drop technology, liquid overlay technology or magnetic 3D bioprinting techniques.

Hydrogels for 3D Cell Culture

“The effect of matrix stiffness on the phenotype and differentiation pathway of mesenchymal stem cells (MSCs) that differentiated to neural, myogenic, or osteogenic phenotypes depending on the elasticity and stiffness of the 3D culture matrix. Stiffer gels favored an osteogenic phenotype while softer gels favored a neural phenotype and aligned scaffolds enable basic research into cell behavior” (Pek et al. (2010)).

mimsys G

mimsys G

Adipose Tissue derived Stem Cells in mimsys G

Adipose Tissue derived Stem Cells in mimsys G

Our partner irisbiosciences, a spin-off of Stemmatters (Portugal), offers hydrogels and adipose tissue derived cells asresearch grade and GMP products.

mimsys G is a defined and animal-free hydrogel consists of methacrylated gellan gum.

The main characteristics are:

  • Defined & animal-free
  • High Transparency
  • Injectable
  • Allows oxygen and nutrient flow
  • Gelation in less than 5 min by ionic crosslink
  • Customize hydrogel stiffness

mimsys G product information download

mimsys G protocol handbook download

Further available hydrogels:

  • Human collagen hydrogel
  • Bovine collagen hydrogel
  • Rat collagen hydrogel
  • Human Fibrinogen

Scaffolds for 3D Cell Culture

Breast Tissue on AK-PolyFibers™

Breast Tissue on AK-PolyFibers™

AK-Polyfibers™ 3D Cell Culture is Akron Biotech’s new range of 3D cell culture scaffolds made from electrospun polymeric nanofibers. Cultivate your cells on a substrate that closely resembles the mechanical and physical features of natural ECM.

AK-Polyfibers™ is available as random or aligned cell culture well inserts for 6, 12, 24, 48 and 96-well trays.

Flyer AK-Polyfibers™

Standard material options for the fibers include:

  • Poly (e-caprolactone)
  • Collagen

Akron Biotech´s Nanofiber Scaffold Webinar

Scaffold based 3D Systems (Alvetex)

Alvetex post-seeding

Alvetex post-seeding

Alvetex® is a highly porous polystyrene scaffold designed for 3D cell culture.

For further information please check out the webpage of ReproCell!

3D Cell Culture as simple as standard 2D Culture
Our partner CelenysAG has developed a propriety technology to produce Hyaluronic Acid Scaffolds for 3D Cell Culture.



The Hyaluronic Acid Scaffolds comes in a 96 wellformat and are packed as 12, 24 or 96 wells. To use the product you only need to seed your cells on top of the scaffolds and to wait for 1 hour. Then you can add your medium to the well and incubate the 3D cell cultures in your incubator. Celenys has established protocols for nucleic acid extraction, protein extraction and fluorescence labelling. It is also easy to recover the cells after culture in Biomimesys.

Already many cell lines, primary cells and stem cells are used for 3D Cell Culture in Biomimesys.

PB-FP-HA24-1 BIOMIMESYS – HA Scaffolds in 96 well format 1 plate (24 scaffolds/plate)
PB-FP-HA48-1 BIOMIMESYS – HA Scaffolds in 96 well format 1 plate (48 scaffolds/plate)
PB-FP-HA96-1 BIOMIMESYS – HA Scaffolds in 96 well format 1 plate (96 scaffolds/plate)

Download the Biomimsys flyer!

Just watch the following video to realize how easy it is to use Biomimsys.

Magnetic 3D Bioprinting

Rapid, predictive models for biomedical research

High throughput just got faster with our 384 well format! 1536 well format on its way

Our partner n3D Biosciences, offers products centered around their core technology of magnetic 3D cell culture. This process begins with the magnetization of cells by statically incubating them with NanoShuttleTM-PL overnight. The next day, the cells can be aggregated with magnetic forces, either by levitation or printing, to form structurally and biologically representative 3D models in vitro. The advantages of magnetic cell culture include:

• Rapid (15 min – 6 h) 3D model formation for high throughput
• No specialized equipment, media, or artificial substrate
• Magnetization of 3D models makes it easy to handle/retrieve
• Dark nanoparticles make tissues easy to visualize and image
• No effect of NanoShuttleTM-PL on viability, proliferation, inflammatory stress
• No interference of NanoShuttleTM-PL on fluorescence or other experimental techniques (Western blotting, qRT-PCR, viability, etc.)

Magnetic Levitation

Available are 3D Bioprinting systems in different formats:
Magnetic Levitation for research applications:
• Single Well
• 6 Well
• 24 Well
Bioprinting for High Throughput
• 96 Well
• 384 Well
• 1536 Well format is coming soon!

The Bio Assay combines 3D Cell Culture with high throughput & high content testing.
Compounds effects (shrinkage of shperoids) are captured using a compact imaging kit (n3Dock) with an iPodTM programmed by a freely available app (Experimental Assistant) to image whole plates at specific intervals.

n3Dock-iPod based Imaging

High-throughput compound screening in 3D your benefits:
• Biocompatible nanoparticles(1) to magnetize cells
• 3D spheroids/rings mimicking native tissue environments
• Rapid printing of spheroids/rings (15 min to a few hours)(2)
• Shrinkage is label-free, quantitative
• Automated imaging – set iPod and go!
• High-throughput, high-content
• Magnetized spheroids that are easy to handle/retrieve
• No special equipment or media needed

(1)Souza, G. R. et al. Three-dimensional tissue culture based on magnetic cell levitation. Nat. Nanotechnol. 5, 291–6 (2010).
(2)Timm, D. M. et al. A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis. Sci. Rep. 3, 3000 (2013)

Product Information on:
Magnetic 3D Bioprinting
384 Well Bioprinting Kit

Check out n3D publications, whitepapers and posters.

Download the Manuals:
Single Well, 6 Well, 24 Well,
96 Well and 384 Well
Bio Assay

Cell Culture Shop/Search

Contact Us

phone +49(0)89517 771 40
fax +49(0)89 517 771 60

Our Partners

cryo_shop Our partnership with CryoShop gives you the choice to work together with experts in cryopreservation and sample handling.